nprB
168
extracellular neutral protease B, required for the function of the [protein|330CB6A25181F7AFD3C63F73CA21A3428882D100|yitM] toxin
locus
BSU_11100
Molecular weight
59.17 kDa
pI
5.32
function
protection of B. subtilis biofilms against competitors
product
extracellular neutral protease B
essential
no
synonyms
nprB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3227 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,186,037 1,187,653
The protein
Protein family
peptidase M4 family (with [protein|309EAC680ABFF0B736E01F0B9BDFBB2FCBDB1BBF|nprE], according to UniProt)
Structure
[PDB|5A3Y] (thermolysin, 44% identity) [pubmed|26527148]
[AF|P39899]
Paralogous protein(s)
[protein|309EAC680ABFF0B736E01F0B9BDFBB2FCBDB1BBF|nprE]
[wiki|Localization]
secreted (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|57139471EB143F6DCCF092DAD0FBB43B0D50D948|nprB]
description
[pubmed|22383849]
regulatory mechanism
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: activation, [pubmed|31622331], in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]
Biological materials
Mutant
KO7 (''nprE aprE epr mpr nprB vpr bpr''), available as BGSC 1A1133
BKE11100 ([gene|57139471EB143F6DCCF092DAD0FBB43B0D50D948|nprB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAACACCACATCCTTCCT, downstream forward: _UP4_TGAGCAAACAAAAACAGTCA
BKK11100 ([gene|57139471EB143F6DCCF092DAD0FBB43B0D50D948|nprB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAACACCACATCCTTCCT, downstream forward: _UP4_TGAGCAAACAAAAACAGTCA
References
Reviews
Original Publications
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Time of last update: 2024-10-15 20:01:21
Author of last update: Jstuelk