pucI
168
allantoin permease
locus
BSU_36470
Molecular weight
53.82 kDa
pI
7.88
function
purine utilization
product
allantoin permease
essential
no
synonyms
pucI, ywoE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1953 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,752,280 3,753,752
The protein
Catalyzed reaction/ biological activity
uptake of allantoin [Pubmed|26967546]
Protein family
purine-cytosine permease (2.A.39) family (with [protein|CB969A2D60F106466605052299F2A5FF5A20E9ED|yxlA], according to UniProt)
Structure
[PDB|2JLN] (from Microbacterium liquefaciens, 27% identity) [pubmed|18927357]
[AF|P94575]
[wiki|Localization]
cell membrane [Pubmed|26967546]
Expression and Regulation
Operons
genes
[gene|5681A5F186AD4B8C81B78FD9DDAE2C0624BE7CAF|pucI]
description
[Pubmed|12029039]
regulation
expression during spore [wiki|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]
regulatory mechanism
[protein|52C1601482C26400A524E880334BB801F832D6ED|pucR]: activation, [Pubmed|12029039], in [regulon|protein:52C1601482C26400A524E880334BB801F832D6ED|pucR regulon]
Biological materials
Mutant
MGNA-A897 (ywoE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/897 NBRP B. subtilis, Japan]
BKE36470 ([gene|5681A5F186AD4B8C81B78FD9DDAE2C0624BE7CAF|pucI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGCCGGCTCCCCTTTCC, downstream forward: _UP4_TAAGATCTATTTAGACGGTA
BKK36470 ([gene|5681A5F186AD4B8C81B78FD9DDAE2C0624BE7CAF|pucI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGCCGGCTCCCCTTTCC, downstream forward: _UP4_TAAGATCTATTTAGACGGTA
BP1261 ([gene|5681A5F186AD4B8C81B78FD9DDAE2C0624BE7CAF|pucI]::cat trp+) available in [wiki|Jörg Stülke]'s & [wiki|Fabian Commichau]'s lab
References
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Time of last update: 2024-12-21 14:45:35
Author of last update: Melvin.boenninger