recN

recN
168

DNA repair and genetic recombination

locus
BSU_24240
Molecular weight
64.31 kDa
pI
4.8
Protein length
Gene length
function
DNA repair and genetic recombination
product
DNA integrity scanning protein
essential
no
synonyms
recN

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0497 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,520,557  2,522,287
Phenotypes of a mutant
reduced survival after [category|SW.3.1.1|DNA replication] arrest imposed by inhibition of [protein|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC] activity [pubmed|36574412]
The protein
Catalyzed reaction/ biological activity
RecN stimulates polymerizing activity of [protein|64C6D783FF3F41C81B216F798A1DC8071345B1ED|pnpA] [Pubmed|21859751]
Protein family
recN family (single member, according to UniProt)
Structure
[PDB|4AD8] (from Deinococcus radiodurans, 30% identity) [pubmed|23085075]
[AF|P17894]
[wiki|Localization]
nucleoid (multiple) [Pubmed|16479537]
evenly distributed in growing cells [Pubmed|21859751]
upon DNA damage, [protein|5680A06A74D3EC6310EF8677795511C75DB3059E|recN] forms a single focus per nucleoid [Pubmed|21859751], recruitment is affected by the presence of [protein|A3A2EF3C95B833A11843553107D781EDEFF0BC41|fumarase] and depends on [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] and [protein|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[protein|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]-mediated end resection [pubmed|34339298,29140245]
Expression and Regulation
Operons
genes
[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]-[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]
description
[Pubmed|2106508]
regulation
by [protein|search|sRNA] [protein|search|sr1]
additional information
expression is fourfold increased upon depletion of ''[wiki|nusA]'' [http://www.nature.com/articles/nmicrobiol20157 Reference]
Open in new tab

[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]→[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]

2024-09-17 11:54:48

ghost

113

cd9e4583995139b45dec8ee7a4f468ed7762fec6

18832E313ED1EB02E9A9AEB9ED804FDAE6EBBAAD

genes
[gene|EA9FCF84AE4FA80993566B62A9200D4B32AF5670|dxs]-[gene|923C4177500E9678A0B4855F8F481D4A36ECD863|tlyA]-[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]-[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]
description
[pubmed|22383849]
regulation
[protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
regulatory mechanism
stringent response: negative regulation, [pubmed|11948165], in [regulon|other_regulator:stringent response|stringent response]
Open in new tab

[gene|EA9FCF84AE4FA80993566B62A9200D4B32AF5670|dxs]->[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]

2024-09-25 07:52:30

Bzhu

125

b0947a7d3105a0852956b3f944c4db191022c978

B8B8483360C3BE8FDAC3C4C5B3BC2ECD2877E42D

Biological materials
Mutant
1A891 ( ''recN''::''cat''), [Pubmed|8510642], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A891&Search=1A891 BGSC]
BKE24240 ([gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACAGCATTACACCTCTT,  downstream forward: _UP4_TAAGCTGCGCGAGAAGCGCA
BKK24240 ([gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24240 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CAAACAGCATTACACCTCTT,  downstream forward: _UP4_TAAGCTGCGCGAGAAGCGCA
labs
[wiki|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]
References
Reviews
19308706,21517913,22933559,23380520,32286623
Original Publications
15849320,19060143,16385024,17999999,2106508,19730681,16479537,21859751,24373815,15186413,8510642,29140245,23085075,30192981,30401797,34339298,36574412

5680A06A74D3EC6310EF8677795511C75DB3059E

Page visits: 3545

Time of last update: 2024-10-07 07:01:14

Author of last update: Jstuelk