pksC
168
bacillaene synthase trans-acting acyltransferase
locus
BSU_17100
Molecular weight
32.31 kDa
pI
5.33
function
polyketide (bacillaene) synthesis
product
bacillaene synthase trans-acting acyltransferase
essential
no
synonyms
pksC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0331 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,783,763 1,784,629
The protein
Catalyzed reaction/ biological activity
holo-[ACP] + malonyl-CoA --> CoA + malonyl-[ACP] (according to UniProt)
Protein family
fabD family (with [protein|BB8542A6444564B21FF5DB6E2C75192C6DA2DFB8|fabD] and [protein|CB0B856B22984DD5B10F01DD07C3961FAA742923|pksE], according to UniProt)
Structure
[PDB|5DZ6]
[AF|O34825]
Expression and Regulation
Operons
genes
[gene|55C821F68B2E5F9F45CC2B2C9494D48D5C33266A|pksC]-[gene|F3E9CA9330394FD829D59B0C2D4D1EEDD2C22D3B|pksD]-[gene|CB0B856B22984DD5B10F01DD07C3961FAA742923|pksE]-[gene|6BEC9E2BCECE33803EAC14DE7E8E988FC9C8DC8B|acpK]-[gene|85D6E439529E8B3F73244A0B004B394B4FE65509|pksF]-[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]-[gene|A4CF68F602273CFDFD55EFD2C1164F0E2E8F6972|pksH]-[gene|F437A90A97CF8ED4BA1E5CCA8A33D9E93612902B|pksI]-[gene|13EB6B903088C11D985DDB7CEC864797058FE038|pksJ]-[gene|BABBC5848F9BBF9E9318252F5AD7D1C4F55FCEAB|pksL]-[gene|40779D3A6E4F72EEFD702BF9FA15B57951783D12|pksM]-[gene|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|pksN]-[gene|85213235F7D3D8E711963E53F314ED758B5FA94B|pksR]
description
[Pubmed|22383849]
regulation
expressed during the transition from growth to stationary phase ([protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB], [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]) [Pubmed|24187085]
heterogeneous expression, percentage of expressing cells increases during colonization of plant roots [pubmed|34557426]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|24187085], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: activation, [Pubmed|24187085], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
additional information
this is a very large operon comprising about 75 kb
genes
[gene|647B1D7E496E81E7FC7FD2BDCACC2486852BAC19|pksB]-[gene|55C821F68B2E5F9F45CC2B2C9494D48D5C33266A|pksC]-[gene|F3E9CA9330394FD829D59B0C2D4D1EEDD2C22D3B|pksD]-[gene|CB0B856B22984DD5B10F01DD07C3961FAA742923|pksE]-[gene|6BEC9E2BCECE33803EAC14DE7E8E988FC9C8DC8B|acpK]-[gene|85D6E439529E8B3F73244A0B004B394B4FE65509|pksF]-[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]-[gene|A4CF68F602273CFDFD55EFD2C1164F0E2E8F6972|pksH]-[gene|F437A90A97CF8ED4BA1E5CCA8A33D9E93612902B|pksI]-[gene|13EB6B903088C11D985DDB7CEC864797058FE038|pksJ]-[gene|BABBC5848F9BBF9E9318252F5AD7D1C4F55FCEAB|pksL]-[gene|40779D3A6E4F72EEFD702BF9FA15B57951783D12|pksM]-[gene|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|pksN]-[gene|85213235F7D3D8E711963E53F314ED758B5FA94B|pksR]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-A016 (pksC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/16 NBRP B. subtilis, Japan]
BKE17100 ([gene|55C821F68B2E5F9F45CC2B2C9494D48D5C33266A|pksC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCTCAAAGCCACCCT, downstream forward: _UP4_TAAAGCATTTGCCCCTATGG
BKK17100 ([gene|55C821F68B2E5F9F45CC2B2C9494D48D5C33266A|pksC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCTCAAAGCCACCCT, downstream forward: _UP4_TAAAGCATTTGCCCCTATGG
References
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Time of last update: 2025-03-17 00:55:22
Author of last update: Jstuelk