addB
168
ATP-dependent deoxyribonuclease (subunit B), required for efficient survival and replication restart after replication-transcription conflicts, responsible for end resection during dsDNA break repair
locus
BSU_10620
Molecular weight
134.41 kDa
pI
5.39
function
DNA repair/ recombination
product
ATP-dependent deoxyribonuclease (subunit B))
essential
no
synonyms
addB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3857 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,136,320 1,139,820
Phenotypes of a mutant
reduced survival after mitomycin treatment [pubmed|34339298]
''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[gene|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]
The protein
Catalyzed reaction/ biological activity
the enzyme is functional as a heterodimer of the [protein|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA] and [protein|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB] subunits, that it is a rapid and processive DNA helicase, and that it catalyses DNA unwinding using one single-stranded DNA motor of 3'5' polarity located in the [protein|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA] subunit [Pubmed|21071401]
the [protein|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB] subunit contains a second putative ATP-binding pocket, but this does not contribute to the observed helicase activity and may instead be involved in the recognition of recombination hotspot sequences [Pubmed|21071401]
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
Protein family
[wiki|helicase family] (according to UniProt)
[wiki|Domains]
[wiki|UvrD-like helicase ATP-binding domain](aa 1-278) (according to UniProt)
[wiki|UvrD-like helicase C-terminal domain] (aa 281-586) (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
Structure
[PDB|3U4Q] (the [protein|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[protein|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]-DNA complex) [Pubmed|22307084]
[AF|P23477]
Expression and Regulation
Operons
genes
[gene|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]-[gene|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[gene|4D89BFFB1073407A7A1762AFD18B49F3414D6787|sbcD]-[gene|ED825D5B3290BA8F9C92477B1FCC433ADB3C8449|sbcC]-[gene|74E2AB77354355549D14152D3566C9B953A4CB31|hlpB]
description
[Pubmed|22383849]
regulation
positive control by [protein|search|ComK] [Pubmed|7746142]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7746142], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP1106 ([gene|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[gene|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB], spc), available in [wiki|Jörg Stülke]'s lab [pubmed|22178973]
BKE10620 ([gene|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATTAGAAGACCCCTCTC, downstream forward: _UP4_TGGATAAAAAAGGAGGCGGA
BKK10620 ([gene|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATTAGAAGACCCCTCTC, downstream forward: _UP4_TGGATAAAAAAGGAGGCGGA
labs
[wiki|Mark Dillingham], Bristol, U.K. ([http://www.bris.ac.uk/biochemistry/research/md.html homepage])
References
Reviews
Original Publications
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Time of last update: 2024-12-29 19:42:45
Author of last update: Jstuelk