proB
168
glutamate 5-kinase
locus
BSU_13120
Molecular weight
39.30 kDa
pI
6.01
function
biosynthesis of proline
product
glutamate 5-kinase
essential
no
ec
2.7.2.11
synonyms
proB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0263 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,378,496 1,379,593
Phenotypes of a mutant
auxotrophic for proline [Pubmed|21784929]
The protein
Catalyzed reaction/ biological activity
ATP + L-glutamate --> ADP + L-glutamyl 5-phosphate (according to UniProt)
Protein family
glutamate 5-kinase family (with [protein|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ], according to UniProt)
[wiki|Domains]
PUA domain (aa 276-353) (according to UniProt)
Structure
[PDB|2J5V] (from E. coli, 40% identity) [pubmed|17321544]
[AF|P39820]
Effectors of protein activity
the enzymatic activity is feedback-inhibited by proline {PubMed|16958849}}
Paralogous protein(s)
[protein|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|5592C5163CF024289B850916516E2C8C7AD43136|proB]-[gene|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|proA]
description
[Pubmed|21233158]
regulation
induced by proline limitation ([wiki|T-box]) [Pubmed|21233158]
expression requires functional [protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM] [pubmed|30355672]
regulatory mechanism
T-box: RNA switch, in [regulon|other_regulator:T-box|T-box]
[protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]: unknown, [pubmed|30355672], in [regulon|protein:F4097349A563503468A2A14F062AEAC532C7917A|rnpM regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21233158], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
[protein|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|proA] is absent from the membrane proteome of a '[protein|A01637E1BFABA20A2923BA85A25CCD8A0D665F78|bdbC]-[protein|CED818CEF5B573A40F382157375E60C0B331F098|bdbD]' mutant [PubMed|22540663]
the [wiki|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
Biological materials
Mutant
BKE13120 ([gene|5592C5163CF024289B850916516E2C8C7AD43136|proB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATTCTCCTCCGCGGC, downstream forward: _UP4_GTAAAAGACTAGGAGGCGGA
BKK13120 ([gene|5592C5163CF024289B850916516E2C8C7AD43136|proB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATTCTCCTCCGCGGC, downstream forward: _UP4_GTAAAAGACTAGGAGGCGGA
References
Reviews
Original Publications
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Time of last update: 2025-01-28 10:38:49
Author of last update: Melvin.boenninger