rbgA
168
assembly of the 50S subunit of the [wiki|ribosome]
locus
BSU_16050
Molecular weight
31.83 kDa
pI
9.53
function
[category|SW.3.3.1.6|Ribosome assembly]
product
GTPase
essential
yes
synonyms
rbgA, ylqF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1161 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,676,532 1,677,380
Phenotypes of a mutant
essential [Pubmed|12682299]
depletion confers a slow growth phenotype, and induces an abnormality of the ''in vivo'' [wiki|ribosome] profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes [Pubmed|24335279,23700310]
premature 45S particles accumulated in RbgA-depleted cells lack several proteins, including [protein|C5CAED1256F082E722EBA0A72647AA043B5681DB|L16], [protein|4626C659D6F1A81CCC94030F1CBAF7FEC3DE19CA|L27], [protein|A58851E0B5C4DE4D6F3C16FF8C009001E393C3B8|L28], [protein|D73FC9CAD929919050837824B4794F9FC9E83052|L33], [protein|7F22743ED669DBCD4089EA2F86B8FFD924F8C2B9|L35] and [protein|F69B475C014087C6DEBE2F50F91862A623D21D1E|L36] [Pubmed|24335279,23700310]
reduced GTPase activity of [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA] results in the acquisition of [protein|20485A785D3AF82889F2018D3EA5B5FA33E64E95|rplF] suppressor mutations [pubmed|35141754]
several 23S rRNA modifications are missing upon [gene|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA] depletion [pubmed|38765983]
The protein
Catalyzed reaction/ biological activity
proper positioning of [protein|20485A785D3AF82889F2018D3EA5B5FA33E64E95|L6] in the large subunit of the [wiki|ribosome] prior to incorporation of [protein|C5CAED1256F082E722EBA0A72647AA043B5681DB|L16] [Pubmed|25330043]
unlocks the conformation adopted by 23S rRNA helices H97 and H42, which is imposed by the entry of [protein|20485A785D3AF82889F2018D3EA5B5FA33E64E95|uL6] [pubmed|35141754]
binds and hydrolyzes GTP and readily exchanges GDP for GTP
stabilizes rRNA helices in the A and P functional sites [pubmed|31665744]
Protein family
TRAFAC class YlqF/YawG GTPase family (together with [protein|519D4694B2895EE6F0D38D918F52F27734506848|cpgA] and [protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH], according to UniProt)
[wiki|Domains]
RNA-binding domain at the C-terminus [Pubmed|23325847]
CP-type G domain (aa 14-178) (according to UniProt)
Structure
[PDB|1PUJ]
[PDB|6PVK] (complex of RbgA bound to ribosomal 50S subunit assembly intermediate) [pubmed|31665744]
[AF|O31743]
Effectors of protein activity
GTPase activity requires binding of [protein|C5CAED1256F082E722EBA0A72647AA043B5681DB|L16] to the 50S subunit of the [wiki|ribosome] [Pubmed|24335279]
binds ppGpp [Pubmed|26951678], ppGpp inhibits activity [Pubmed|30366986,26951678]
Paralogous protein(s)
[protein|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF], [protein|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg], [protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH], [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA], [protein|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|engA], [protein|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF], [protein|CB68B3393CA7862C45C624AF7B1ADD188243A01F|era], [protein|DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C|ysxC]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA]-[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB]-[gene|0D4A4A1B2FD9D0698D0A65010A8ABC84DA7386A2|ylqG]-[gene|3F271D9FFE4F30D845319CD71662695AA592EC8F|ylqH]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-B141 (ylqF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1140 NBRP B. subtilis, Japan]
labs
[wiki|Naotake Ogasawara], Nara, Japan
References
Reviews
Original Publications
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Time of last update: 2024-11-15 07:54:46
Author of last update: Jstuelk