opuAC
168
glycine betaine and arsenobetaine [wiki|ABC transporter] (binding protein)
locus
BSU_03000
Molecular weight
32.06 kDa
pI
8.02
function
compatible solute transport
product
glycine betaine and arsenobetaine [wiki|ABC transporter] (binding protein)
essential
no
synonyms
opuAC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2113 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
323,119 324,000
The protein
Catalyzed reaction/ biological activity
binding for subsequent uptake of glycine betaine and arsenobetaine [pubmed|29159878,24561588]
binding for subsequent uptake of dimethylglycine [Pubmed|24561588]
Structure
[PDB|2B4L] (complex with glycine betaine)
[PDB|3CHG] (in complex with DMSA)
[PDB|5NXX] (in complex with arsenobetaine) [pubmed|29159878]
[AF|P46922]
[wiki|Localization]
associated to the membrane (via [protein|5F989C57010ACFC03E37AF5A894153F432520921|opuAB]) [Pubmed|10092453]
Expression and Regulation
Operons
genes
[gene|FB2220730013A98D7C979062A4A451518B92DF09|opuAA]-[gene|5F989C57010ACFC03E37AF5A894153F432520921|opuAB]-[gene|546407EFD8128D1F74443991B8D600F668FBD7A1|opuAC]
description
[Pubmed|22383849]
regulation
induced by osmotic stress [Pubmed|23175650]
regulatory mechanism
[protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]: activation, [Pubmed|23646920], in [regulon|protein:6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23175650], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE03000 ([gene|546407EFD8128D1F74443991B8D600F668FBD7A1|opuAC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGCCTATGATTTTTTTAA, downstream forward: _UP4_TAATCAAAAAAGCAGCCTGT
BKK03000 ([gene|546407EFD8128D1F74443991B8D600F668FBD7A1|opuAC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGCCTATGATTTTTTTAA, downstream forward: _UP4_TAATCAAAAAAGCAGCCTGT
Expression vectors
pGP2926 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-31 02:43:10
Author of last update: Jstuelk