gltR
168
transcriptional regulator ([wiki|LysR family])
locus
BSU_26670
Molecular weight
32.79 kDa
pI
6.65
function
unknown
product
transcriptional regulator ([wiki|LysR family])
essential
no
synonyms
gltR, yrdL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0583 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,725,837 2,726,727
Phenotypes of a mutant
The function of this protein is unknown. However, certain mutant forms are able to activate expression of the [gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB] operon (and thus to replace the cognate activator protein [protein|87BCAE725B02860156D50E1783F6DB68510C811E|gltC]) [pubmed|9023181,28294562]
The protein
Protein family
[wiki|LysR family] (according to UniProt)
[wiki|Domains]
[wiki|HTH lysR-type domain] (aa 1-58) (according to UniProt)
Structure
[PDB|5Z72] (B. amyloliquefaciens [protein|FE15A41A58A8177280817CA3825764C39185021A|ccpC], 22% identity)
[AF|P94501]
Additional information
The function of this protein is unknown. However, certain mutant forms are able to activate expression of the [gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB] operon (and thus to replace the cognate activator protein [protein|87BCAE725B02860156D50E1783F6DB68510C811E|gltC]) [pubmed|9023181,28294562]
information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000049 PRODORIC2 database]
Expression and Regulation
Operons
genes
[gene|544CE1370BE7444F361F62808E320CE97C3C2F93|gltR]
description
(according to [http://dbtbs.hgc.jp/COG/prom/gltR.html DBTBS]) null
regulatory mechanism
[protein|544CE1370BE7444F361F62808E320CE97C3C2F93|gltR]: negative autoregulation, in [regulon|protein:544CE1370BE7444F361F62808E320CE97C3C2F93|gltR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9023181], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP3615 (spec), available in [wiki|Jörg Stülke]'s lab [pubmed|36377869]
GP345 (erm), available in [wiki|Jörg Stülke]'s lab
BKE26670 ([gene|544CE1370BE7444F361F62808E320CE97C3C2F93|gltR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE26670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAGTCCTCCATAGTTG, downstream forward: _UP4_TAAATAAATGGATGGAAGGC
BKK26670 ([gene|544CE1370BE7444F361F62808E320CE97C3C2F93|gltR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK26670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAGTCCTCCATAGTTG, downstream forward: _UP4_TAAATAAATGGATGGAAGGC
Expression vectors
pBP421 (N-terminal Strep-tag, for [wiki|SPINE], purification from ''B. subtilis'', in [wiki|pGP382]) (available in [wiki|Fabian Commichau]'s lab)
labs
[wiki|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
References
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Time of last update: 2025-01-20 06:52:35
Author of last update: Jstuelk