pucJ
168
uric acid permease
locus
BSU_32430
Molecular weight
46.94 kDa
pI
9.64
function
purine utilization
product
uric acid permease
essential
no
synonyms
pucJ, yunJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2233 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,330,502 3,331,851
The protein
Protein family
[wiki|xanthine/uracil permease family] (according to UniProt)
[wiki|Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily] (according to UniProt)
Structure
[PDB|3QE7] (E. coli uracil transporter, 31% identity) [pubmed|21423164]
[AF|O32139]
Paralogous protein(s)
[protein|7D56F85FBD3AAC66607DD7465592EEAE09D61C98|pbuX], [protein|3162EF36F4441A1E4EBBFDAD19F6768D8EF21B29|pucK]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|53950BFDC128DE46F3F43BDB9D0FDB3D645152DF|pucJ]-[gene|3162EF36F4441A1E4EBBFDAD19F6768D8EF21B29|pucK]-[gene|2E7DEC53A130247E66225E87950D702E462C324F|pucL]-[gene|A2F93FFE225DFDBCBB3EA079BEF84C79465F0E5C|pucM]
description
[Pubmed|12029039]
regulation
expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]
regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|12823818], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|52C1601482C26400A524E880334BB801F832D6ED|pucR]: activation, [Pubmed|12029039], in [regulon|protein:52C1601482C26400A524E880334BB801F832D6ED|pucR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12029039], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|52C1601482C26400A524E880334BB801F832D6ED|pucR]-[gene|53950BFDC128DE46F3F43BDB9D0FDB3D645152DF|pucJ]-[gene|3162EF36F4441A1E4EBBFDAD19F6768D8EF21B29|pucK]-[gene|2E7DEC53A130247E66225E87950D702E462C324F|pucL]-[gene|A2F93FFE225DFDBCBB3EA079BEF84C79465F0E5C|pucM]
description
[Pubmed|12823818]
regulation
expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]
regulatory mechanism
[protein|52C1601482C26400A524E880334BB801F832D6ED|pucR]: auto-repression, [Pubmed|12029039], in [regulon|protein:52C1601482C26400A524E880334BB801F832D6ED|pucR regulon]
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|25755103], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
Biological materials
Mutant
MGNA-A935 (yunJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/935 NBRP B. subtilis, Japan]
BKE32430 ([gene|53950BFDC128DE46F3F43BDB9D0FDB3D645152DF|pucJ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTATAGTCCCTCCCTGTA, downstream forward: _UP4_TTAGAAAAAGAGGTGTAAAA
BKK32430 ([gene|53950BFDC128DE46F3F43BDB9D0FDB3D645152DF|pucJ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTATAGTCCCTCCCTGTA, downstream forward: _UP4_TTAGAAAAAGAGGTGTAAAA
BP1257([gene|search|pucJK]::cat trp+) available in [wiki|Jörg Stülke]'s & [wiki|Fabian Commichau]'s lab
References
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Time of last update: 2024-12-21 05:40:34
Author of last update: Melvin.boenninger