metK
168
S-adenosylmethionine synthetase
locus
BSU_30550
Molecular weight
43.88 kDa
pI
5.03
function
S-adenosylmethionine synthesis
product
S-adenosylmethionine synthetase
essential
yes
ec
2.5.1.6
synonyms
metK, metE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0192 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,127,825 3,129,027
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
ATP + H2O + L-methionine --> diphosphate + phosphate + S-adenosyl-L-methionine (according to UniProt)
Protein family
AdoMet synthase family (single member, according to UniProt)
Structure
[PDB|1FUG] (from ''Escherichia coli'', 63% identity, 75% similarity) [Pubmed|8723769]
[AF|P54419]
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|52D4DA0AA45F0774543786B55D18DF9DF53A5029|metK]-[gene|6F5BBCBA02EA1C016AA37EB63D252A4C07CE6617|asnB]-[gene|219586A3F378DC38EA076FD39B99D41136BDE722|alaP]
description
[Pubmed|33155333]
regulation
constitutive
genes
[gene|52D4DA0AA45F0774543786B55D18DF9DF53A5029|metK]
description
[Pubmed|9387221]
regulation
induced by methionine starvation ([wiki|S-box]) [Pubmed|10094622]
regulatory mechanism
S-box: RNA switch, the [wiki|S-box] [wiki|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|other_regulator:S-box|S-box]
Biological materials
lacZ fusion
pGP432 (in [wiki|pAC7]), available in [wiki|Jrg Stlke]'s lab
References
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Time of last update: 2024-12-26 08:36:56
Author of last update: Melvin.boenninger