glgB
168
1,4-alpha-glucan branching enzyme
locus
BSU_30980
Molecular weight
73.49 kDa
pI
6.24
function
glycogen biosynthesis
product
1,4-alpha-glucan branching enzyme
essential
no
ec
2.4.1.18
synonyms
glgB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0296 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,169,763 3,171,646
The protein
Catalyzed reaction/ biological activity
Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain (according to UniProt)
Protein family
[wiki|glycosyl hydrolase 13 family] (according to UniProt)
Structure
[PDB|5GQU] (from Cyanothece sp., 46% identity) [pubmed|28193843]
[AF|P39118]
Paralogous protein(s)
[protein|7D783DD180D950A4B030BF5EA78CCE930A466A07|amyX]
Expression and Regulation
Operons
genes
[gene|52C8DE3C78B680C8883D1CD559CBC2D9ABFCC400|glgB]-[gene|B665735B76462724A3C7A1E89D3E613C18F21399|glgC]-[gene|63A4FD35A2E72C665BFA3F2632F06C7A0C954087|glgD]-[gene|EBEA85AD543B79C321CB5F28D1B7E1248640DC1D|glgA]-[gene|3AB4513126EFF54996A54CB7DEB6062C1A06AAC3|glgP]
description
[Pubmed|8145641]
regulation
expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|8145641]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|8145641], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
BKE30980 ([gene|52C8DE3C78B680C8883D1CD559CBC2D9ABFCC400|glgB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAATCATCCTTTCTGA, downstream forward: _UP4_AAAAAAAGAGGAGAGATAAA
BKK30980 ([gene|52C8DE3C78B680C8883D1CD559CBC2D9ABFCC400|glgB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAATCATCCTTTCTGA, downstream forward: _UP4_AAAAAAAGAGGAGAGATAAA
References
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Time of last update: 2024-12-30 11:46:46
Author of last update: Melvin.boenninger