cpgA
168
ribosome assembly GTPase, activity stimulated by ribosomes, may be involved in the maturation of the 30S subunit of the ribosome, detoxification of 4-phosphoerythronate
locus
BSU_15780
Molecular weight
33.64 kDa
pI
4.74
function
ribosome assembly, coordination of peptidoglycan deposition in the cell wall, metabolite proofreading
product
GTPase
essential
no
synonyms
cpgA, yloQ, rsgA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1162 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,653,103 1,653,999
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
forms curly cells [Pubmed|22544754]
poor growth [pubmed|28189581]
non-transformable [pubmed|28189581]
defective in 16S rRNA 3' processing by [protein|E0437C86E5F3707FF1DA13C8B98F73130DED8FB4|yqfG] [pubmed|31003868]
severe growth inhibition upon addition of subinhibitory concentrations of mirubactin C [pubmed|36312962]
The protein
Catalyzed reaction/ biological activity
dephosphorylation of 4-phosphoerythronate thus preventing inhibition of [protein|61B7C51EB7E74226890010B61D8E41C02189A453|gndA] [pubmed|30948730]
Protein family
TRAFAC class YlqF/YawG GTPase family (together with [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA] and [protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH], according to UniProt)
[wiki|Domains]
CP-type G domain (aa 67-228) (according to UniProt)
Structure
[PDB|1T9H] [pubmed|15223319]
[AF|O34530]
Modification
''in vitro'' phosphorylated on Thr-166 by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC], dephosphorylated by [protein|84447F9A6644EA8A7593BB99B2B69D4377E670E2|prpC] [Pubmed|22544754,19246764]
Expression and Regulation
Operons
genes
[gene|84447F9A6644EA8A7593BB99B2B69D4377E670E2|prpC]-[gene|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC]-[gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]-[gene|8103B8539338B77CF51B16B7FA76783ED2319F13|rpeA]-[gene|0ACE023DCD6A6BB5A1827D1D997BDFAC3B168C51|yloS]
description
[Pubmed|22383849]
genes
[gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]
description
[Pubmed|16025310]
expression
expression is decreased upon depletion of depletion of tRNA maturation factors [gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB] or [gene|C40C5D35ED53D343C8200248FCCB010BAB388054|rnz] [pubmed|36840557]
Biological materials
Mutant
MGNA-B135 (yloQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1134 NBRP B. subtilis, Japan]
BKE15780 ([gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15780 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTCAGGCATATTTTCCCTCC, downstream forward: _UP4_GACAGAAAGCCGAGGTATTA
BKK15780 ([gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15780 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTCAGGCATATTTTCCCTCC, downstream forward: _UP4_GACAGAAAGCCGAGGTATTA
BP1272 ([gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]::cat trp+) available in [wiki|Jörg Stülke]'s & [wiki|Fabian Commichau]'s lab, strain cannot grow with glucose, see Sachla et al (2019)
labs
[wiki|Tony Wilkinson], York University, U.K. [http://www.york.ac.uk/depts/chem/staff/ajw.html homepage]
References
Reviews
Original Publications
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Time of last update: 2024-10-10 04:26:04
Author of last update: Christoph.elfmann