cpgA

cpgA
168

ribosome assembly GTPase, activity stimulated by ribosomes, may be involved in the maturation of the 30S subunit of the ribosome, detoxification of 4-phosphoerythronate

locus
BSU_15780
Molecular weight
33.64 kDa
pI
4.74
Protein length
Gene length
function
ribosome assembly, coordination of peptidoglycan deposition in the cell wall, metabolite proofreading
product
GTPase
essential
no
synonyms
cpgA, yloQ, rsgA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1162 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,653,103  1,653,999
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
forms curly cells [Pubmed|22544754]
poor growth [pubmed|28189581]
non-transformable [pubmed|28189581]
defective in 16S rRNA 3' processing by [protein|E0437C86E5F3707FF1DA13C8B98F73130DED8FB4|yqfG] [pubmed|31003868]
severe growth inhibition upon addition of subinhibitory concentrations of mirubactin C [pubmed|36312962]
The protein
Catalyzed reaction/ biological activity
dephosphorylation of 4-phosphoerythronate thus preventing inhibition of [protein|61B7C51EB7E74226890010B61D8E41C02189A453|gndA] [pubmed|30948730]
Protein family
TRAFAC class YlqF/YawG GTPase family (together with [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA] and [protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH], according to UniProt)
[wiki|Domains]
CP-type G domain (aa 67-228) (according to UniProt)
Structure
[PDB|1T9H] [pubmed|15223319]
[AF|O34530]
Modification
''in vitro'' phosphorylated on Thr-166 by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC], dephosphorylated by [protein|84447F9A6644EA8A7593BB99B2B69D4377E670E2|prpC] [Pubmed|22544754,19246764]
Expression and Regulation
Operons
genes
[gene|84447F9A6644EA8A7593BB99B2B69D4377E670E2|prpC]-[gene|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC]-[gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]-[gene|8103B8539338B77CF51B16B7FA76783ED2319F13|rpeA]-[gene|0ACE023DCD6A6BB5A1827D1D997BDFAC3B168C51|yloS]
description
[Pubmed|22383849]
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[gene|84447F9A6644EA8A7593BB99B2B69D4377E670E2|prpC]→[gene|0ACE023DCD6A6BB5A1827D1D997BDFAC3B168C51|yloS]

2024-11-14 08:57:39

Jstuelk

116

f45e3810755c3fba238243b21c1c17e072c8fe47

14D2827FA6BD60210DFADC1BB1FFF0C98DC768FA

genes
[gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]
description
[Pubmed|16025310]
expression
expression is decreased upon depletion of depletion of tRNA maturation factors [gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB] or [gene|C40C5D35ED53D343C8200248FCCB010BAB388054|rnz] [pubmed|36840557]
Open in new tab

[gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]

2024-11-07 09:59:12

Jstuelk

89

9B5376A22F42C49A349590080432E7A657E40BDC

A15AF0C53EF5258846124085281D05395B8422FD

Biological materials
Mutant
MGNA-B135 (yloQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1134 NBRP B. subtilis, Japan]
BKE15780 ([gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15780 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTCAGGCATATTTTCCCTCC,  downstream forward: _UP4_GACAGAAAGCCGAGGTATTA
BKK15780 ([gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15780 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTCAGGCATATTTTCCCTCC,  downstream forward: _UP4_GACAGAAAGCCGAGGTATTA
BP1272 ([gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]::cat trp+) available in [wiki|Jörg Stülke]'s & [wiki|Fabian Commichau]'s lab, strain cannot grow with glucose, see Sachla et al (2019)
labs
[wiki|Tony Wilkinson], York University, U.K. [http://www.york.ac.uk/depts/chem/staff/ajw.html homepage]
References
Reviews
19575570
Original Publications
15223319,16485133,14747714,22544754,18344364,19246764,19246764,15828870,17005971,18344364,18984160,28189581,30948730,31003868,36312962,36840557

519D4694B2895EE6F0D38D918F52F27734506848

Page visits: 5383

Time of last update: 2024-12-04 18:54:27

Author of last update: Christoph.elfmann