feuA
168
[wiki|ABC transporter] for the siderophores Fe-enterobactin and Fe-bacillibactin (binding protein), with [protein|5C52DB31F378E49AABE5D937DD901A68F3810477|yusV] as ATPase
locus
BSU_01630
Molecular weight
34.95 kDa
pI
8.02
function
[wiki|acquisition of iron]
product
[wiki|ABC transporter] for the siderophores Fe-enterobactin and Fe-bacillibactin (binding protein) [pubmed|28283524]
essential
no
synonyms
feuA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0614 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
182,370 183,323
Phenotypes of a mutant
severe growth inhibition upon addition of subinhibitory concentrations of mirubactin C [pubmed|36312962]
The protein
Protein family
[wiki|Bacterial solute-binding protein 8 family] (according to UniProt)
[wiki|Domains]
Two independent non-symmetric globular domains, connected by a long alpha-helix (residues 143-164 of the mature protein).
[wiki|Fe/B12 periplasmic-binding domain] (aa 57-317) (according to UniProt)
Structure
[PDB|2PHZ], [PDB|2WI8], [PDB|2WHY] (complex with ferri-bacillibactin)
[PDB|2XUZ] (complex with ferri-enterobactin)
[PDB|2XV1] (complex with ferric mecam)
[AF|P40409]
Modification
FeuA is a lipoprotein with a N-acetyl-S-diacyl-glyceryl-cysteine structure [Pubmed|22303020]
[wiki|Localization]
associated to the membrane (lipoprotein) [Pubmed|22303020]
extracellular (signal peptide) [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|516B13F337FD346B4A4A268E35D1EBABB05957E1|feuA]-[gene|57402D137E3D3791CAF0696421224F4E1DC1BA48|feuB]-[gene|F38F6D4841B3044EA0496B3A1C1D484018BDFEA4|feuC]-[gene|745167427618C4DA71848F9362043B66BC4E392E|ybbA]
description
[Pubmed|12354229]
regulation
expression during spore [wiki|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]
induced by iron starvation (second wave to allow iron scavenging from the environment) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [pubmed|29133393]
regulatory mechanism
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
[protein|EB28A65CECE994DF2DF486DEACF40F2533703DB0|btr]: activation, in the presence of the co-activators bacillibactin or enterobactin [Pubmed|17725565], in [regulon|protein:EB28A65CECE994DF2DF486DEACF40F2533703DB0|btr regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|17725565], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the presence of an iron-responsive element bound by [wiki|CitB] between ''[wiki|feuA]'' and ''[wiki|feuB]'' suggests iron-dependent regulation by [wiki|CitB] [Pubmed|10468622]
Other regulations
[protein|E15E9F88B0E03FE8834C646369D2F4B4E713EF41|citB]: translation control
Biological materials
Mutant
BKE01630 ([gene|516B13F337FD346B4A4A268E35D1EBABB05957E1|feuA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATAGAGCCTCCTGTT, downstream forward: _UP4_AACTAATTCAGAGTAGGTTT
BKK01630 ([gene|516B13F337FD346B4A4A268E35D1EBABB05957E1|feuA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATAGAGCCTCCTGTT, downstream forward: _UP4_AACTAATTCAGAGTAGGTTT
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-12-31 02:48:20
Author of last update: Jstuelk