yrvO

yrvO
168

cysteine desulfurase, sulfur transfer to [protein|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU], involved in 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules

locus
BSU_27510
Molecular weight
41.03 kDa
pI
5.46
Protein length
Gene length
function
tRNA modification
product
cysteine desulfurase
essential
yes
ec
2.8.1.7
synonyms
nifS, iscS, iscS1, YrvO

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1104 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,810,559  2,811,698
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules (together with [protein|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]) [Pubmed|25825430]
U34 in tRNA + L-cysteine + ATP --> (s2U34 in tRNA) + alanine + AMP + ppi [Pubmed|25825430]
Protein family
[wiki|Class-V pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Cofactors]
PLP [pubmed|38187551]
Structure
[PDB|1EG5] (from Thermotoga maritima, 37% identity, 54% similarity) [Pubmed|10715213]
[AF|O34599]
Modification
phosphorylated on Arg-385 [Pubmed|22517742]
Paralogous protein(s)
[protein|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|ycbU], [protein|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ], [protein|88ACE6B79534338F7C72F107B35A0B2384007088|sufS], [protein|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|nifS]
Expression and Regulation
Operons
genes
[gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]-[gene|07DFF7D7BE1BACFB148F600400BDE5933BF1DD07|yrrB]-[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]
description
[Pubmed|22383849]
Open in new tab

[gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]→[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]

2024-10-11 04:37:15

Jstuelk

104

abda92d242629b6aea524d43804e9037e28674a8

021F54A200AB704BB82EBD13945C64A7665E25EA

genes
[gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]
description
[Pubmed|16885442]
regulation
[protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16885442], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]→[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]

2024-10-11 21:28:06

ghost

130

8c74dd15c62e3038ffc334dd6a5d07145ed7c2d8

DE1A6CEF81820F1A8EA3E9CEE9ECE501D6C33477

Biological materials
Mutant
MGNA-A828 (yrvO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/828 NBRP B. subtilis, Japan]
References
Reviews
34201508
Original Publications
16885442,11948165,10715213,22517742,12682299,17064282,25825430,35467390,38187551

4F5945C3C8F18BD1B30D97CD516A06077C200B9D

Page visits: 3581

Time of last update: 2024-10-14 02:24:35

Author of last update: Jstuelk