yrvO
168
cysteine desulfurase, sulfur transfer to [protein|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU], involved in 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules
locus
BSU_27510
Molecular weight
41.03 kDa
pI
5.46
function
tRNA modification
product
cysteine desulfurase
essential
yes
ec
2.8.1.7
synonyms
nifS, iscS, iscS1, YrvO
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1104 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,810,559 2,811,698
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules (together with [protein|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]) [Pubmed|25825430]
U34 in tRNA + L-cysteine + ATP --> (s2U34 in tRNA) + alanine + AMP + ppi [Pubmed|25825430]
Protein family
[wiki|Class-V pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Cofactors]
PLP [pubmed|38187551]
Structure
[PDB|1EG5] (from Thermotoga maritima, 37% identity, 54% similarity) [Pubmed|10715213]
[AF|O34599]
Modification
phosphorylated on Arg-385 [Pubmed|22517742]
Paralogous protein(s)
[protein|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|ycbU], [protein|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ], [protein|88ACE6B79534338F7C72F107B35A0B2384007088|sufS], [protein|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|nifS]
Expression and Regulation
Operons
genes
[gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]-[gene|07DFF7D7BE1BACFB148F600400BDE5933BF1DD07|yrrB]-[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]
description
[Pubmed|22383849]
genes
[gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]
description
[Pubmed|16885442]
regulation
[protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16885442], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A828 (yrvO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/828 NBRP B. subtilis, Japan]
References
Reviews
Original Publications
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Time of last update: 2024-12-27 05:36:39
Author of last update: Jstuelk