maeA
168
malic enzyme, required for malate dependent catabolite repression of the [gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]-[gene|F6C3B267106E7510FABECF0B13ABF222901689A9|pftB] operon
locus
BSU_37050
Molecular weight
63.93 kDa
pI
4.88
function
malate utilization
product
NAD-dependent malate dehydrogenase
essential
no
ec
1.1.1.38
synonyms
maeA, ywkA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0281 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,800,418 3,802,166
The protein
Catalyzed reaction/ biological activity
(S)-malate + NAD+ --> CO2 + NADH + pyruvate (according to UniProt)
H+ + oxaloacetate --> CO2 + pyruvate (according to UniProt)
Protein family
[wiki|malic enzymes family] (according to UniProt)
[wiki|Cofactors]
NAD+
Structure
[PDB|1LLQ] (from Ascaris suum, 41% identity) [pubmed|12033925]
[AF|P45868]
Paralogous protein(s)
[protein|BBBCD5F56779895189E21076AF165B901F654534|malS]
Expression and Regulation
Operons
genes
[gene|4F30A4412768E6313F57BD56C4EF5079F500E0C4|maeA]-[gene|1E602BCB2A416CDBF2B5007765EC9628123FE979|ywkB]
description
[Pubmed|12949160]
regulation
expressed in the presence of malate ([protein|search|MalR]) [Pubmed|12949160]
regulatory mechanism
[protein|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR]: activation, [Pubmed|12949160], in [regulon|protein:689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12949160], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
Biological materials
Mutant
MGNA-B657 (ywkA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1656 NBRP B. subtilis, Japan]
GP1141 (aphA3) available in [wiki|Jörg Stülke]'s lab
BKE37050 ([gene|4F30A4412768E6313F57BD56C4EF5079F500E0C4|maeA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAAATATGACCGATC, downstream forward: _UP4_TAATTAGCGAGTAAATTTTT
BKK37050 ([gene|4F30A4412768E6313F57BD56C4EF5079F500E0C4|maeA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAAATATGACCGATC, downstream forward: _UP4_TAATTAGCGAGTAAATTTTT
labs
[wiki|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
References
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Time of last update: 2025-04-03 18:07:36
Author of last update: Melvin.boenninger