cssS
168
two-component sensor kinase, control of cellular responses to protein secretion stress
locus
BSU_33020
Molecular weight
51.92 kDa
pI
5.8
function
control of cellular responses to protein secretion stress
product
two-component sensor kinase
essential
no
synonyms
cssS, yvqB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0642 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,386,398 3,387,753
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [protein|A40CD2C23A19860342F440284302EFFDAC09E88A|cssR]
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
[wiki|Domains]
two transmembrane segments, C-terminal histidine phosphotransferase domain
[wiki|HAMP domain] (aa 287-239) (according to UniProt)
[wiki|Histidine kinase domain] (aa 247-451) (according to UniProt)
Structure
[AF|O32193]
Modification
autophosphorylation on a His residue
Effectors of protein activity
the extracellular loop domain is required for signal perception [Pubmed|22307758]
Paralogous protein(s)
[protein|52BB660B0CAB36C74F1D47963235846B45AF78AB|yclK], [protein|96E4470EF4B558F4EC318FEBF58B779BA50DB7E2|yvrG], [protein|9E8A6AB3920BFBFDAAD1F2E4F333A32354ABB25B|ykoH], [protein|1582E81F7DA85F38D6732D341ABD0F052587F25A|kinE]
[wiki|Localization]
cell membrane (according to Swiss-Prot)
localized primarily at the division septum but also found in a punctate pattern with lower intensity throughout the cell cylinder [Pubmed|22307758]
Expression and Regulation
Operons
genes
[gene|A40CD2C23A19860342F440284302EFFDAC09E88A|cssR]-[gene|4EE48E4931F662E51586DB6D01E91C688329222D|cssS]
description
[Pubmed|11555295]
regulation
expressed under conditions of secretion stress ([protein|search|CssR]) [Pubmed|12270824]
regulatory mechanism
[protein|A40CD2C23A19860342F440284302EFFDAC09E88A|cssR]: activation, [Pubmed|12270824], in [regulon|protein:A40CD2C23A19860342F440284302EFFDAC09E88A|cssR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12270824], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B216 (yvqB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1215 NBRP B. subtilis, Japan]
BKE33020 ([gene|4EE48E4931F662E51586DB6D01E91C688329222D|cssS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATGATGACATCATCCT, downstream forward: _UP4_TAGACTGTAGATGTTTTGCA
BKK33020 ([gene|4EE48E4931F662E51586DB6D01E91C688329222D|cssS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATGATGACATCATCCT, downstream forward: _UP4_TAGACTGTAGATGTTTTGCA
References
Reviews
Original Publications
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Time of last update: 2024-10-08 11:41:21
Author of last update: Jstuelk