hmoB
168
heme monooxygenase
locus
BSU_10100
Molecular weight
18.68 kDa
pI
5.22
function
degradation of heme, acquisition of iron
product
heme monooxygenase
essential
no
ec
null
synonyms
hmoB, yixC, yhgC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2329 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,083,229 1,083,729
The protein
Catalyzed reaction/ biological activity
binds hemin ''in vitro'' with ~1:1 stoichiometry and degrade hemin in the presence of an electron donor [Pubmed|21873409]
heme b + 3 O2 + 3 reduced [NADPH—hemoprotein reductase] --> biliverdin IXα + CO + Fe2+ + H+ + 3 H2O + 3 oxidized [NADPH—hemoprotein reductase] (according to UniProt)
Protein family
antibiotic biosynthesis monooxygenase family (with [protein|A4E1A1FA886980C863919DA011AC1EEDDBD74967|hmoA], according to UniProt)
[wiki|Domains]
[wiki|ABM domain] (aa 66-153) (according to UniProt)
Structure
[PDB|3TVZ] [Pubmed|22531134]
[AF|P38049]
Expression and Regulation
Operons
genes
[gene|4EE1809AC405170A78F6F8C8C02EEF5CC54DE076|hmoB]
description
[Pubmed|8335642]
regulation
constitutively expressed [Pubmed|21873409]
Biological materials
Mutant
MGNA-B503 (yhgC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1502 NBRP B. subtilis, Japan]
BKE10100 ([gene|4EE1809AC405170A78F6F8C8C02EEF5CC54DE076|hmoB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCAGCCACCTCCTGCT, downstream forward: _UP4_TAGCTGCCGGCATGTCCGGC
BKK10100 ([gene|4EE1809AC405170A78F6F8C8C02EEF5CC54DE076|hmoB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCAGCCACCTCCTGCT, downstream forward: _UP4_TAGCTGCCGGCATGTCCGGC
References
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Time of last update: 2024-10-08 23:20:58
Author of last update: Jstuelk