ftsH
168
ATP-dependent metalloprotease
locus
BSU_00690
Molecular weight
70.76 kDa
pI
5.84
function
cell division, sporulation initiation
product
ATP-dependent metalloprotease
essential
no
synonyms
ftsH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0465 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
76,984 78,897
Phenotypes of a mutant
loss of [wiki|genetic competence] [pubmed|33897624]
defective in biofilm formation [Pubmed|22882210]
strongly reduced sporulation frequency [Pubmed|22142536], [Pubmed|26735940]
The protein
Catalyzed reaction/ biological activity
degrades [protein|A574974B6F4FF46DC69E03AE021651C67089866F|spo0E] and [protein|86BEE9B4DEA83B3B8EFC52EFDDC1F62314FC4291|spo0M], resulting in reduced sporulation frequency in a ''ftsH'' mutant [Pubmed|22142536]
[protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH] is involved in the degradation of [protein|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA] [Pubmed|24222488]
degrades [protein|7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A|spoIIE] [Pubmed|26465112]
Protein family
central section: [wiki|AAA ATPase family] (according to UniProt)
C-terminal section: Peptidase M41 family (single member, according to UniProt)
Structure
[PDB|1LV7] (from ''E. coli'', 64% identity) [Pubmed|12176385]
[AF|P37476]
Effectors of protein activity
protease activity is controlled by the interaction with the flottilins [protein|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA] and [protein|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [Pubmed|24222488]
Paralogous protein(s)
[protein|E278CCD5CFD618A0D4ACB92E1E06DCC1B4570ED0|yjoB]
[wiki|Localization]
cell membrane [Pubmed|18763711]
preferentially at the division septum [Pubmed|22882210]
forms some foci in the membrane [Pubmed|22882210]
Expression and Regulation
Operons
genes
[gene|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]-[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]-[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
description
[Pubmed|18179421]
regulation
induced by heat shock (class III)
regulatory mechanism
[protein|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]: activation, with [protein|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT] [Pubmed|24001521], in [regulon|protein:473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS regulon]
[protein|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]: activation, with [protein|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS] [Pubmed|24001521], in [regulon|protein:B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT regulon]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7608085], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
genes
[gene|7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A|spoIIE]-[gene|C8AA53889FC8B389E1D921B762BD78990C7A8E57|yabS]-[gene|E86A96B832351FF513DD9853EAD8998CC44C9951|yabT]-[gene|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]-[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]-[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
description
[pubmed|22383849]
regulation
expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|16497325]
sigma factors
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
genes
[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
description
[Pubmed|7608085]
regulation
induced by heat shock (class III)
additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
Biological materials
Mutant
GP2640 (Δ[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]::aphA3) available in [wiki|Jörg Stülke]'s lab [pubmed|33897624]
MGNA-A084 (ftsH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/84 NBRP B. subtilis, Japan]
1A1061 ( ''ftsH''::''cat''), [Pubmed|9076729], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1061&Search=1A1061 BGSC]
1A1060 ( ''ftsH''::''erm''), [Pubmed|10851010], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1060&Search=1A1060 BGSC]
BKE00690 ([gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCTTACCTCCTCCCAC, downstream forward: _UP4_TAATTCGCTTTCTTTCTAAA
BKK00690 ([gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCTTACCTCCTCCCAC, downstream forward: _UP4_TAATTCGCTTTCTTTCTAAA
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-11-21 12:48:19
Author of last update: Dennis.wicke