ftsH

ftsH
168

ATP-dependent metalloprotease

locus
BSU_00690
Molecular weight
70.76 kDa
pI
5.84
Protein length
Gene length
function
cell division, sporulation initiation
product
ATP-dependent metalloprotease
essential
no
synonyms
ftsH

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0465 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
76,984  78,897
Phenotypes of a mutant
loss of [wiki|genetic competence] [pubmed|33897624]
defective in biofilm formation [Pubmed|22882210]
strongly reduced sporulation frequency [Pubmed|22142536], [Pubmed|26735940]
The protein
Catalyzed reaction/ biological activity
degrades [protein|A574974B6F4FF46DC69E03AE021651C67089866F|spo0E] and [protein|86BEE9B4DEA83B3B8EFC52EFDDC1F62314FC4291|spo0M], resulting in reduced sporulation frequency in a ''ftsH'' mutant [Pubmed|22142536]
[protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH] is involved in the degradation of [protein|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA] [Pubmed|24222488]
degrades [protein|7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A|spoIIE] [Pubmed|26465112]
Protein family
central section: [wiki|AAA ATPase family] (according to UniProt)
C-terminal section: Peptidase M41 family (single member, according to UniProt)
Structure
[PDB|1LV7] (from ''E. coli'', 64% identity) [Pubmed|12176385]
[AF|P37476]
Effectors of protein activity
protease activity is controlled by the interaction with the flottilins [protein|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA] and [protein|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [Pubmed|24222488]
Paralogous protein(s)
[protein|E278CCD5CFD618A0D4ACB92E1E06DCC1B4570ED0|yjoB]
[wiki|Localization]
cell membrane [Pubmed|18763711]
preferentially at the division septum [Pubmed|22882210]
forms some foci in the membrane [Pubmed|22882210]
Expression and Regulation
Operons
genes
[gene|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]-[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]-[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
description
[Pubmed|18179421]
regulation
induced by heat shock (class III)
regulatory mechanism
[protein|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]: activation, with [protein|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT] [Pubmed|24001521], in [regulon|protein:473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS regulon]
[protein|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]: activation, with [protein|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS] [Pubmed|24001521], in [regulon|protein:B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT regulon]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7608085], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
Open in new tab

[gene|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]→[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]

2024-10-07 10:54:42

ghost

86

0b831aee45fd6cc4e3e06cbf53fe7330f12b4cb1

0B2B3D2B74921E6EEF5704482CF1D3DF309FA3D3

genes
[gene|7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A|spoIIE]-[gene|C8AA53889FC8B389E1D921B762BD78990C7A8E57|yabS]-[gene|E86A96B832351FF513DD9853EAD8998CC44C9951|yabT]-[gene|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]-[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]-[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
description
[pubmed|22383849]
regulation
expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|16497325]
sigma factors
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
Open in new tab

[gene|7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A|spoIIE]->[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]

2024-10-06 06:35:50

Jstuelk

104

7069328a463a18ae1e3c27f03ddf76f79efe7c06

8FBB4B976CF46DAD9437300BBE1B22B618D33ED0

genes
[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
description
[Pubmed|7608085]
regulation
induced by heat shock (class III)
additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
Open in new tab

[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]

2024-10-11 11:54:45

ghost

98

7e04f0d15d662d6dfeefd5537cf2a5e7778fc26c

DFE2E933DDBA16A1B214B7C055E64EAA42F028A5

Biological materials
Mutant
GP2640 (Δ[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]::aphA3) available in [wiki|Jörg Stülke]'s lab [pubmed|33897624]
MGNA-A084 (ftsH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/84 NBRP B. subtilis, Japan]
1A1061 ( ''ftsH''::''cat''), [Pubmed|9076729], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1061&Search=1A1061 BGSC]
1A1060 ( ''ftsH''::''erm''), [Pubmed|10851010], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1060&Search=1A1060 BGSC]
BKE00690 ([gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00690 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCCTTACCTCCTCCCAC,  downstream forward: _UP4_TAATTCGCTTTCTTTCTAAA
BKK00690 ([gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00690 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCCTTACCTCCTCCCAC,  downstream forward: _UP4_TAATTCGCTTTCTTTCTAAA
References
Reviews
23479438,26639779,28748186,34410368,35638784
Original Publications
10913836,12533473,9076729,10851010,9084181,15386101,10077851,19332814,12884008,7608085,9352926,19332814,18763711,12176385,18179421,24001521,22882210,22142536,24222488,26297017,26465112,26735940,31930742,33897624,36815589

4E7B9426CED372AA8A321A147116A3A589FBF20C

Page visits: 8028

Time of last update: 2024-10-12 16:18:44

Author of last update: Jstuelk