kapD
168
3'-exoribonuclease, inhibitor of the [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA] pathway to sporulation
locus
BSU_31470
Molecular weight
23.68 kDa
pI
6.38
function
regulation of sporulation initiation
product
3'-exoribonuclease, inhibitor of the [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA] pathway to sporulation
essential
no
ec
null
synonyms
kapD, yugB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5018 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,231,812 3,232,429
The protein
Catalyzed reaction/ biological activity
RNA degradation, e.g. the [gene|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK] mRNA [pubmed|39817517]
Protein family
DEDDh family of 3′-exoribonucleases [pubmed|39817517]
[wiki|Domains]
[wiki|Exonuclease domain] (aa 6-173) (according to UniProt)
Structure
[AF|O05231]
[wiki|Localization]
assembles onto the surface of the developing spore [pubmed|39817517]
cytoplasm (Homogeneous) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|4E384490E7D2761BC8779483C4950D6D14323D1C|kapD]
description
[pubmed|22383849]
regulation
expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE], [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]) [pubmed|39817517]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [pubmed|39817517], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [pubmed|39817517], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
MGNA-A611 (kapD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/611 NBRP B. subtilis, Japan]
BKE31470 ([gene|4E384490E7D2761BC8779483C4950D6D14323D1C|kapD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTTTTACACCTGCCTTT, downstream forward: _UP4_TAAAAAACCCAATCTTTTGT
BKK31470 ([gene|4E384490E7D2761BC8779483C4950D6D14323D1C|kapD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTTTTACACCTGCCTTT, downstream forward: _UP4_TAAAAAACCCAATCTTTTGT
References
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Time of last update: 2025-03-16 17:48:49
Author of last update: Jstuelk