glmS

glmS
168

[metabolite|glutamine]-[metabolite|fructose-6-phosphate] transaminase

locus
BSU_01780
Molecular weight
65.16 kDa
pI
4.8
Protein length
Gene length
function
cell wall synthesis
product
[metabolite|glutamine]-[metabolite|fructose-6-phosphate] transaminase
essential
yes
ec
2.6.1.16
synonyms
glmS, gcaA, ybxD

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0449 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
200,277  202,079
Phenotypes of a mutant
essential [Pubmed|12682299]
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The protein
Catalyzed reaction/ biological activity
D-[metabolite|fructose-6-phosphate] + L-[metabolite|glutamine] --> D-[metabolite|glucosamine-6-phosphate] + L-[metabolite|glutamate] (according to UniProt)
[wiki|Domains]
[wiki|Glutamine amidotransferase type-2 domain] (aa 2-217) (according to UniProt)
[wiki|SIS domain] 1 (aa 283-422) (according to UniProt)
[wiki|SIS domain] 2 (aa 452-590) (according to UniProt)
Structure
[PDB|2VF4] (GlmS from ''E. coli'', 39% identity, 58% similarity) [Pubmed|18295797]
the ribozyme: [PDB|3G8S], [PDB|3G9C], [PDB|3G8T], [PDB|3G95], [PDB|3G96] (all for the ribozyme from ''Bacillus anthracis''), [PDB|2HO7] (the ribozyme from ''Thermonanaerobacter tengcongensis'')
[AF|P0CI73]
Effectors of protein activity
activity is stimulated by interaction with  [protein|7150BABA5086D5E2EB8102E4901A216F43576282|glmR] [pubmed|32994436,30248093]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
Expression and Regulation
Operons
genes
[gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]-[gene|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR]-[gene|F022ACB2CBB8DA46B392CE1D26474093645F26DA|glmM]-[gene|4E23D2043D51C587027ABBF6335F148295E90B8F|glmS]
description
[Pubmed|23192352]
regulatory mechanism
GlmS ribozyme: RNA switch, in [regulon|other_regulator:GlmS ribozyme|GlmS ribozyme]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22211522], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]→[gene|4E23D2043D51C587027ABBF6335F148295E90B8F|glmS]

2024-06-02 18:53:46

Jstuelk

110

d0e388855d436414f58a0fe3c7427dc65a267c07

DAD2D234926A5513FEB7883AAB4D651657324B25

additional information
''glmS'' is only expressed in the absence of glucosamine 6-phosphate ([wiki|GlmS ribozyme])
glucosamine 6-phosphate binds the leader mRNA, and a [wiki|riboswitch] with [wiki|ribozyme] activity cleaves off the [gene|4E23D2043D51C587027ABBF6335F148295E90B8F|glmS] section from the mRNA, resulting in stopp of transcript elongation
labs
[wiki|Wade Winkler], University of Texas, USA, [http://www.utsouthwestern.edu/findfac/professional/0,,68018,00.html Homepage]
References
Reviews
18279655,24769284,12859215,20709015,12044898,34248920,37238589
Original Publications
14343123,17981983,11160890,18295797,20525796,23192352,23876412,22211522,30105781,30248093,32994436,38723147
The ''glmS'' Ribozyme
18079181,16484375,16784238,15096624,16990543,17114942,16484375,15029187,17283212,16298301,19228039,21317896,21395279,24318896,28192411,38000388

4E23D2043D51C587027ABBF6335F148295E90B8F

Page visits: 7067

Time of last update: 2024-07-15 00:31:40

Author of last update: Jstuelk