yhdN
168
general stress protein, broad specificity aldo-keto reductase that converts MG to acetol
locus
BSU_09530
Molecular weight
37.16 kDa
pI
4.77
function
detoxification of methylglyoxal
product
aldo/keto reductase, specific for NADP
essential
no
synonyms
yhdN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0667 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,030,265 1,031,260
Phenotypes of a mutant
increased sensitivity to methylglyoxal [Pubmed|24330391]
The protein
Catalyzed reaction/ biological activity
methylglyoxal ---> acetol [Pubmed|24330391]
Protein family
[wiki|Aldo/keto reductase family] (according to UniProt)
[wiki|Cofactors]
NADPH [Pubmed|24330391]
Structure
[PDB|1PZ1] [Pubmed|15019785]
[AF|P80874]
Paralogous protein(s)
[protein|B73D729097C00BE6F3C7FF1708738783EE93C6FB|yccK], [protein|D0A2506FDCEA41D2A8910AC2837B46E0B311D63B|ycsN], [protein|F1AFFACD36C75702B0BB8F0B4241A9688FB6253C|iolS]
Expression and Regulation
Operons
genes
[gene|4DF9B105ABA64AFD5106EE75FCC9EFAB52BEFC83|yhdN]-[gene|FC05D5FC95CEDC4B4A04040C86170D185528B9FF|plsC]
description
[Pubmed|10220166]
regulation
induced by stress ([protein|search|SigB]) [Pubmed|10220166,15805528]
regulatory mechanism
[protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]: repression, [Pubmed|12737802], in [regulon|protein:FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR regulon]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|10220166,15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Mutant
MGNA-A695 (yhdN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/695 NBRP B. subtilis, Japan]
BKE09530 ([gene|4DF9B105ABA64AFD5106EE75FCC9EFAB52BEFC83|yhdN]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTGCCACTCCTTCACT, downstream forward: _UP4_TAACAAAAGCTGAGGGCGAA
BKK09530 ([gene|4DF9B105ABA64AFD5106EE75FCC9EFAB52BEFC83|yhdN]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTGCCACTCCTTCACT, downstream forward: _UP4_TAACAAAAGCTGAGGGCGAA
References
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Time of last update: 2024-12-20 00:39:52
Author of last update: Jstuelk