gamP
168
glucosamine-specific permease of the [wiki|phosphotransferase systems|phosphotransferase system], EIICBA of the [category|SW.1.2.2|PTS]
locus
BSU_02350
Molecular weight
67.97 kDa
pI
5.47
function
glucosamine uptake and phosphorylation
product
glucosamine-specific [category|SW.1.2.2|PTS], EIICBA
essential
no
ec
2.7.1.69
synonyms
gamP, ybfS, yzfA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2190 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
254,907 256,802
Phenotypes of a mutant
delayed growth with glucosamine as the single carbon source [Pubmed|10627040]
The protein
Catalyzed reaction/ biological activity
protein EIIA Npi-phospho-L-histidine + protein EIIB --> protein EIIA + protein EIIB Npi-phospho-L-histidine/cysteine (according to UniProt)
Protein family
[category|SW.1.2.2|PTS] permease, glucose family [Pubmed|10627040]
[wiki|Domains]
[wiki|PTS EIIA domain] type-1 (aa 515-619) (according to UniProt)
[wiki|PTS EIIB domain] type-1 (aa 397-478) (according to UniProt)
[wiki|PTS EIIC domain] type-1 (aa 1-382) (according to UniProt)
Structure
[PDB|6BVG] (EIIC domain from B. cereus, corresponds to aa 5 ... 380), 32.5% identity [pubmed|29784777]
[PDB|3BP3] (EIIB domain from E. coli, corresponds to aa 403 ... 467), 56.9% identity [pubmed|18319344]
[PDB|1AX3] (EIIA domain of [protein|B5E7EB475434E96786C577AE709A21BD702733D8|ptsG], corresponds to aa 489 ... 622), 55.6% identity [pubmed|9593197]
[AF|P39816]
Paralogous protein(s)
[protein|7D5A8084FDDA962CCF028FC3735E68C4F2E77084|nagP], [protein|B5E7EB475434E96786C577AE709A21BD702733D8|ptsG], [protein|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]
[wiki|Localization]
membrane [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|8BBC9AFF4CD56A3A66E270A2BBA193D095EE01D1|gamA]-[gene|4DF1740D4DFB9FD25E77C14D28AEA01707776099|gamP]
description
[Pubmed|23667565]
regulation
induced by glucosamine ([protein|search|GamR]) [Pubmed|24673833,23667565]
regulatory mechanism
[protein|FB074E8CFCA1C0198457170A7AFD85C875B34DF2|gamR]: repression, [Pubmed|24673833,23667565], in [regulon|protein:FB074E8CFCA1C0198457170A7AFD85C875B34DF2|gamR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23667565], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B937 (ybfS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1936 NBRP B. subtilis, Japan]
QB6098 (aphA3), available in [wiki|Jörg Stülke]'s lab
BKE02350 ([gene|4DF1740D4DFB9FD25E77C14D28AEA01707776099|gamP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACAGTCTCCTTTTATT, downstream forward: _UP4_TAAAAAAGTCCCCCCTGCTG
BKK02350 ([gene|4DF1740D4DFB9FD25E77C14D28AEA01707776099|gamP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACAGTCTCCTTTTATT, downstream forward: _UP4_TAAAAAAGTCCCCCCTGCTG
References
Reviews
Original Publications
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Time of last update: 2024-12-22 01:00:12
Author of last update: Jstuelk