helD
168
DNA 3-5 helicase IV, removes and recycles stalled [wiki|transcription] complexes
locus
BSU_33450
Molecular weight
89.74 kDa
pI
6.08
function
[wiki|transcription]
product
ATP-dependent [wiki|transcription] recycling factor
essential
no
ec
3.6.4.12
synonyms
helD, yvgS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3973 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,434,327 3,436,651
The protein
Catalyzed reaction/ biological activity
removes nucleic acids from the active site of RNA polymerase, and induces a large conformational change in the primary channel of RNA polymerase leading to removal and recycling of the stalled polymerase [pubmed|33339820]
Protein family
[wiki|helicase family] (according to UniProt)
[wiki|Domains]
an N-terminal region (NTR; residues 4–187) [pubmed|33339827]
[wiki|UvrD-like helicase ATP-binding domain] (aa 212-610) consisting of two globular domains (D1a/D1b, residues 188–338/491–603; D2, residues 604–774) [pubmed|33339827]
an elongated helical protrusion in D1 (HelDBumper; residues 339–490) [pubmed|33339827]
Structure
[PDB|3DMN] (C-terminal domain of the protein from ''Lactobacillus plantarum'', 39% identity)
[AF|O32215]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|4DE90422754DA1FD9EFE58766EACB99CE6171207|helD]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A456 (yvgS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/456 NBRP B. subtilis, Japan]
1A899 ( ''helD''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A899&Search=1A899 BGSC]
BKE33450 ([gene|4DE90422754DA1FD9EFE58766EACB99CE6171207|helD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33450 BGSC] as well as in [wiki|Fabian Commichau]'s and [wiki|Jörg Stülke]'s labs, [Pubmed|28189581], upstream reverse: _UP1_CATGAACGGCACCTCCAGAA, downstream forward: _UP4_TGAAGAGAAGGGAGTCCTTC
BKK33450 ([gene|4DE90422754DA1FD9EFE58766EACB99CE6171207|helD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33450 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATGAACGGCACCTCCAGAA, downstream forward: _UP4_TGAAGAGAAGGGAGTCCTTC
References
Reviews
Original Publications
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Time of last update: 2024-12-21 20:43:31
Author of last update: Jstuelk