spoVFB
168
dipicolinate synthase (subunit B)
locus
BSU_16740
Molecular weight
21.72 kDa
pI
6.15
function
dipicolic acid production
product
dipicolinate synthase (subunit B)
essential
no
synonyms
spoVFB, dpaB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5843 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,745,263 1,745,865
The protein
Catalyzed reaction/ biological activity
(S)-2,3-dihydrodipicolinate + NADP+ --> dipicolinate + H+ + NADPH (according to UniProt)
Structure
[PDB|3MCU] (from B. cereus, 72% identity)
[AF|Q04810]
Expression and Regulation
Operons
genes
[gene|3DA80060554B78E9DC2189CECB78680095B08B32|spoVFA]-[gene|4D93A13DB9DC55A49525BFF4FD589F4FF67D50E8|spoVFB]-[gene|564A2F43F8AABE97FC8F0B1B3382A9F31F02FA18|asd]-[gene|6D6718AC72C9D86EDE16B191EDAB3ABA488B689B|dapG]-[gene|00B4CA4E25858A56F9D4E0ADEE2643F203FEC613|dapA]
description
[Pubmed|8098035]
regulation
expressed late during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|15699190,8098035]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190,8098035], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
Biological materials
Mutant
BKE16740 ([gene|4D93A13DB9DC55A49525BFF4FD589F4FF67D50E8|spoVFB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTCCTTTTAATGACGACA, downstream forward: _UP4_TAAATTTACAGAAAATCTTT
BKK16740 ([gene|4D93A13DB9DC55A49525BFF4FD589F4FF67D50E8|spoVFB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTCCTTTTAATGACGACA, downstream forward: _UP4_TAAATTTACAGAAAATCTTT
References
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Time of last update: 2024-12-19 15:34:06
Author of last update: Jstuelk