tlpB
168
membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein, acts also as pH sensor, responds to increasing pH as an attractant signal
locus
BSU_31230
Molecular weight
71.36 kDa
pI
4.73
function
control of chemotaxis, sensing of alkaline environments
product
methyl-accepting chemotaxis protein
essential
no
synonyms
tlpB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0840 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,204,067 3,206,055
The protein
[wiki|Domains]
[wiki|Cache domain] (aa 153-228) (according to UniProt)
[wiki|HAMP domain] (aa 203-355) (according to UniProt)
[wiki|Methyl-accepting transducer domain] (aa 374-610) (according to UniProt)
Structure
[PDB|6S1K] (from E. coli, corresponds to aa 285 ... 616, 29% identity) [pubmed|31925330]
[AF|P39217]
Paralogous protein(s)
[protein|8333CD46F704F03A22482CAA98DEFCC945362A10|mcpC], [protein|E4E1020B799A1B403BDB4847B91B02D64D161AF3|mcpB], [protein|F02E3080214A327E131ECDE0DBC52C57EFDFE2CE|mcpA], [protein|1DE26CDEE952142C1303C822F0E2A63AE09F721B|tlpA]
[wiki|Localization]
cell membrane [Pubmed|21515776]
Expression and Regulation
Operons
genes
[gene|4CD44AD8FE68516C84889EAA2137828E06B8A30B|tlpB]
description
[Pubmed|8188684]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|8188684], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
additional information
in minimal medium, TlpB is present with 2,500 +/- 740 molecules per cell [PubMed|21515776]
Biological materials
Mutant
BKE31230 ([gene|4CD44AD8FE68516C84889EAA2137828E06B8A30B|tlpB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCGTTGACTCCTCCT, downstream forward: _UP4_TAATGAAACGAGAAAGCGGC
BKK31230 ([gene|4CD44AD8FE68516C84889EAA2137828E06B8A30B|tlpB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCGTTGACTCCTCCT, downstream forward: _UP4_TAATGAAACGAGAAAGCGGC
References
Reviews
Original Publications
Page visits: 2212
Time of last update: 2024-10-08 09:18:06
Author of last update: Jstuelk