cca
168
tRNA nucleotidyltransferase, maturation of the single-copy tRNACys, which lacks an encoded CCA 3 end
locus
BSU_22450
Molecular weight
45.29 kDa
pI
8.04
function
tRNA modification
product
tRNA nucleotidyltransferase
essential
yes
ec
2.7.7.25
synonyms
cca, papS, ypjI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0617 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,355,880 2,357,073
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
tRNA precursor + ATP + 2 CTP --> tRNA with 3' CCA end + 3 diphosphate (according to UniProt)
Protein family
tRNA nucleotidyltransferase/poly(A) polymerase family (single member, according to UniProt)
the protein is similar to the ''E. coli'' poly(A) polymerase
Structure
[PDB|1MIV] (Geobacillus stearothermophilus 44% identity)
[AF|P42977]
Expression and Regulation
Operons
genes
[gene|A828298EA2A002538876FBA6113648AAD9C4C317|ypjD]-[gene|5AB90032031653A913E15B26B3E1AE246E95AABF|dapB]-[gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]-[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]-[gene|187C78441816FC74B0FCCE34997E8AEC0A80B114|bshA]-[gene|4C4FA59FF5D100E941EF470C0C54E85681ADC74F|cca]-[gene|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]
description
[Pubmed|20308541]
regulation
induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|23894131], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23894131], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
References
Reviews
Original Publications
Operon and expression
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Time of last update: 2024-10-13 20:18:41
Author of last update: Melvin.boenninger