yrhJ
168
cytochrome P450 (CYP102A3)/ NADPH-cytochrome P450 reductase
locus
BSU_27160
Molecular weight
118.47 kDa
pI
6.02
function
fatty acid metabolism
product
NADPH-cytochrome P450 reductase
essential
no
ec
1.6.2.4
synonyms
yrhJ, cypB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2124 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,773,890 2,777,054
The protein
Catalyzed reaction/ biological activity
hydroxylates medium-chain fatty acids in subterminal positions [Pubmed|14741768]
organic molecule + O2 + reduced [NADPH—hemoprotein reductase] --> alcohol + H+ + H2O + oxidized [NADPH—hemoprotein reductase] (according to UniProt)
NADPH + 2 oxidized [cytochrome P450] --> H+ + NADP+ + 2 reduced [cytochrome P450] (according to UniProt)
Protein family
N-terminal part: [wiki|cytochrome P450] family (according to UniProt)
[wiki|Domains]
[wiki|Flavodoxin-like domain] (aa 486-625) (according to UniProt)
[wiki|FAD-binding FR-type domain] (aa 663-896) (according to UniProt)
Structure
[PDB|2X7Y] (from Bacillus megaterium; 67% identity, 88% similarity) [Pubmed|25325618,22949185]
[AF|O08336]
Paralogous protein(s)
[protein|9AA8CAEB42B1ED4CC8D80D57B045A55307114D6A|yetO]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|3DA3FFB295F5513A220CB163994EE0F356C810E6|fatR]-[gene|4BA5214C441C6671CB7DAA3CACBCA5FC025BE0E6|yrhJ]
description
[Pubmed|10917605]
regulation
expression is reduced in a [protein|search|SigV] mutant [Pubmed|21926231]
regulatory mechanism
[protein|3DA3FFB295F5513A220CB163994EE0F356C810E6|fatR]: repression, [Pubmed|11734890], in [regulon|protein:3DA3FFB295F5513A220CB163994EE0F356C810E6|fatR regulon]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421,12775685], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|12207695], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]
[protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]: sigma factor, [Pubmed|9636707], in [regulon|protein:E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX regulon]
additional information
A [protein|search|ncRNA] ([wiki|RnaC]) is encoded between '[protein|search|yrhK]' and '[protein|search|yrhJ]' [PubMed|25790031]
genes
[gene|6D5089931FBDB398A7FAF3C8DDF341EB4949AEFE|yrhH]-[gene|3DA3FFB295F5513A220CB163994EE0F356C810E6|fatR]-[gene|4BA5214C441C6671CB7DAA3CACBCA5FC025BE0E6|yrhJ]
description
[Pubmed|18179421]
regulation
Expressed under stress conditions ([protein|search|SigW], [protein|search|SigX]) [Pubmed|9683469]
additional information
A [protein|search|ncRNA] ([wiki|RnaC]) is encoded between '[protein|search|yrhK]' and '[protein|search|yrhJ]' [PubMed|25790031]
Biological materials
Mutant
MGNA-A163 (yrhJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/163 NBRP B. subtilis, Japan]
BKE27160 ([gene|4BA5214C441C6671CB7DAA3CACBCA5FC025BE0E6|yrhJ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGTTTCATTAACATCTCCC, downstream forward: _UP4_TAAAATATAAAATCCCGCCA
BKK27160 ([gene|4BA5214C441C6671CB7DAA3CACBCA5FC025BE0E6|yrhJ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGTTTCATTAACATCTCCC, downstream forward: _UP4_TAAAATATAAAATCCCGCCA
References
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Time of last update: 2024-10-07 00:37:53
Author of last update: Melvin.boenninger