ydiG
168
molybdenum cofactor biosynthesis protein
locus
BSU_05960
Molecular weight
18.31 kDa
pI
6.22
function
unknown
product
molybdenum cofactor biosynthesis protein
essential
no
ec
null
synonyms
ydiG, moaC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0315 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
646,582 647,094
The protein
Catalyzed reaction/ biological activity
involved in the first step of molybdenum cofactor biosynthesis (together with [protein|19EB8E7CBC0E8D7A6429ADFFF93D1F71EFE3FF11|moaA])
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate --> cyclic pyranopterin phosphate + diphosphate (according to UniProt)
Protein family
moaC family (single member, according to UniProt)
Structure
[PDB|1EKR] (the protein from ''E. coli'', 51% identity, 78% similarity) [Pubmed|10903949]
[AF|O05520]
Expression and Regulation
Operons
genes
[gene|4A8D74B83E20FA138ED66F0D8FD456B69B740D92|ydiG]-[gene|B5EF521437323EF43F08E5EFDB5C798616CA499A|rex]-[gene|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY]-[gene|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|tatCY]
description
[Pubmed|22383849]
regulation
constitutitvely expressed [Pubmed|22383849]
the mRNA is processed between [gene|B5EF521437323EF43F08E5EFDB5C798616CA499A|rex] and [gene|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
Biological materials
Mutant
MGNA-C200 (ydiG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2198 NBRP B. subtilis, Japan]
BKE05960 ([gene|4A8D74B83E20FA138ED66F0D8FD456B69B740D92|ydiG]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTAAAACGCTCCTTAA, downstream forward: _UP4_GAGTATAATTTGGAGGACCA
BKK05960 ([gene|4A8D74B83E20FA138ED66F0D8FD456B69B740D92|ydiG]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTAAAACGCTCCTTAA, downstream forward: _UP4_GAGTATAATTTGGAGGACCA
References
Reviews
Original Publications
Page visits: 2198
Time of last update: 2025-01-22 22:24:12
Author of last update: Robert.warneke