tgl
168
outer spore coat protein, spore associated transglutaminase
locus
BSU_31270
Molecular weight
28.15 kDa
pI
6.69
function
introduction of crosslinks in the spore coat proteins [protein|1CA990DA55B6F879968C431A703E0478F5564A1A|gerQ] and [protein|CEBEC9CECCF445C40D793E61A2CD23175631A473|safA]
product
spore associated transglutaminase
essential
no
ec
2.3.2.13
synonyms
tgl, yuxF, yugV
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5915 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,212,591 3,213,328
The protein
Catalyzed reaction/ biological activity
cross-links [protein|CEBEC9CECCF445C40D793E61A2CD23175631A473|safA] to other spore coat proteins [pubmed|29712873]
L-glutaminyl-[protein] + L-lysyl-[protein] --> [protein]-L-lysyl-N6-5-L-glutamyl-[protein] + NH4+ (according to UniProt)
temperature-dependent modification of the coat proteins such as [protein|1CA990DA55B6F879968C431A703E0478F5564A1A|gerQ] (together with [protein|A46D07F8F56249A04C5F880120D19F00C9CAE112|yabG]) [Pubmed|16751597]
Protein family
bacillus TGase family (single member, according to UniProt)
Structure
[PDB|4P8I] [Pubmed|26322858]
[PDB|4PA5] (complexed with cystamine) [Pubmed|26322858]
[AF|P40746]
[wiki|Localization]
outer spore coat, localization depends on [protein|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE] [Pubmed|22171814]
inner spore coat and cortex, dependent on [protein|CEBEC9CECCF445C40D793E61A2CD23175631A473|safA] [pubmed|29712873]
Expression and Regulation
Operons
genes
[gene|4A2B1DA38608E5A4096087BF7246D4C64C54DBE3|tgl]
description
[Pubmed|9274030]
regulation
expressed late during sporulation in the mother cell ([protein|search|SigE], [protein|search|SigK], [protein|search|GerE]) [Pubmed|15383836,15699190,12501297]
regulatory mechanism
[protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: repression, [Pubmed|15383836], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190,12501297], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15383836], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
MGNA-A615 (tgl::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/615 NBRP B. subtilis, Japan]
1S122 ( ''tgl''::''erm''), [Pubmed|15317760], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S122&Search=1S122 BGSC]
BKE31270 ([gene|4A2B1DA38608E5A4096087BF7246D4C64C54DBE3|tgl]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTAGCCCCCCTTGTT, downstream forward: _UP4_ATCGTCCGCTAAAAAGCCCC
BKK31270 ([gene|4A2B1DA38608E5A4096087BF7246D4C64C54DBE3|tgl]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTAGCCCCCCTTGTT, downstream forward: _UP4_ATCGTCCGCTAAAAAGCCCC
labs
[wiki|Adriano Henriques], Lisbon, Portugal [http://www.itqb.unl.pt/research/biology/microbial-development homepage]
References
Reviews
Original Publications
Page visits: 3068
Time of last update: 2024-10-07 07:05:31
Author of last update: Melvin.boenninger