mleN
168
[metabolite|malate]-H+/Na+-[metabolite|lactate] antiporter, uptake of D-asparagine
locus
BSU_23560
Molecular weight
50.05 kDa
pI
7.25
function
[metabolite|malate] uptake
product
[metabolite|malate]-H+/Na+-[metabolite|lactate] antiporter
essential
no
synonyms
mleN, yqkI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1757 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,452,800 2,454,206
Phenotypes of a mutant
resistant to otherwise toxic [metabolite|D-asparagine] [pubmed|38193659]
abrupt arrest in the mid-logarithmic phase of growth on [metabolite|malate] when low concentrations of protonophore were present [Pubmed|10903309]
The protein
Catalyzed reaction/ biological activity
uptake of [metabolite|D-asparagine] [pubmed|38193659]
Protein family
NhaC Na(+)/H(+) (TC 2.A.35) antiporter family (with [protein|A7F1B4C0D32E18773170BFBEEB94E5EF55C7DB97|nhaC], according to UniProt)
Structure
[AF|P54571]
Paralogous protein(s)
[protein|A7F1B4C0D32E18773170BFBEEB94E5EF55C7DB97|nhaC]
[wiki|Localization]
membrane associated [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]-[gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]
description
[Pubmed|22383849]
regulation
''[protein|search|ansA]'': expressed in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]
regulatory mechanism
[protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]: repression, [Pubmed|11914346], in [regulon|protein:146947C974D62BCB282BA3F5B924B35695D75886|ansR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|22900538], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1711029], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]-[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]-[gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]
description
[Pubmed|22383849]
regulation
[protein|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]: expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
expression
expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
regulatory mechanism
[protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]: transcription repression, [pubmed|11914346], in [regulon|protein:146947C974D62BCB282BA3F5B924B35695D75886|ansR regulon]
Biological materials
Mutant
GP1460 (Δ[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]::''kan''), available in [wiki|Jörg Stülke]'s lab [pubmed|38193659]
GP4165 (Δ[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]::''kan''), available in [wiki|Jörg Stülke]'s lab
MGNA-C411 (yqkI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2409 NBRP B. subtilis, Japan]
GP1460 (''mleN''::''spc''), available in [wiki|Jörg Stülke]'s lab
BKE23560 ([gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAGAAGTCCCCCTTCAT, downstream forward: _UP4_GGCTAATAGGGGAGGATCAC
BKK23560 ([gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAGAAGTCCCCCTTCAT, downstream forward: _UP4_GGCTAATAGGGGAGGATCAC
lacZ fusion
pGP388 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab [pubmed|38193659]
References
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Time of last update: 2024-12-13 11:32:17
Author of last update: Jstuelk