cotG
168
outer spore coat protein
locus
BSU_36070
Molecular weight
23.81 kDa
pI
10.67
function
control of spore permeability, resistance of the spore
product
spore coat protein
essential
no
synonyms
cotG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
3,717,238 3,717,825
The protein
Catalyzed reaction/ biological activity
required for the maturation of [protein|2A0B2CA7C982C03C1AFBDB3540A918EE937B06CB|cotB] [Pubmed|26953338]
Structure
[AF|P39801]
Modification
phosphorylated on Ser-15, Ser-39, and Thr-147 by [protein|D53067098F693669F61E5CCF6C06664580C40C79|cotH], phosphorylation does not take place at increased temperature [Pubmed|35254711,27185916,25115591]
[wiki|Localization]
outer spore coat, localization depends on [protein|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE] [Pubmed|22171814]
assembly around the forming spore depends on [protein|D53067098F693669F61E5CCF6C06664580C40C79|cotH]-mediated phosphorylation [pubmed|35254711]
accumulation In the mother cell (non-phosphorylated [protein|49BAFE3E69F1F91AEF4154F55B44639DD3CE4A71|cotG]) [pubmed|35254711]
Expression and Regulation
Operons
genes
[gene|49BAFE3E69F1F91AEF4154F55B44639DD3CE4A71|cotG]
description
[Pubmed|7814326]
regulation
expressed late during sporulation in the mother cell ([protein|search|SigE], [protein|search|SigK], [protein|search|GerE]) [Pubmed|15383836,15699190,7814326]
regulatory mechanism
[protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: activation, [Pubmed|7814326], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]
[protein|0D7F5504590512E1598B94DC1734BF8FF67ED994|gerR]: activation, [Pubmed|15621419], in [regulon|protein:0D7F5504590512E1598B94DC1734BF8FF67ED994|gerR regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15383836], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190,7814326], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
Biological materials
Mutant
BKE36070 ([gene|49BAFE3E69F1F91AEF4154F55B44639DD3CE4A71|cotG]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTGAAATCCTCCTTTT, downstream forward: _UP4_TAATCTAAGTTTTCATTGTT
BKK36070 ([gene|49BAFE3E69F1F91AEF4154F55B44639DD3CE4A71|cotG]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTGAAATCCTCCTTTT, downstream forward: _UP4_TAATCTAAGTTTTCATTGTT
References
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Time of last update: 2024-10-10 22:44:52
Author of last update: Jstuelk