ispG
168
similar to peptidoglycan acetylation, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase, 6th step in the MEP pathway of isoprenoid biosynthesis
locus
BSU_25070
Molecular weight
40.42 kDa
pI
6.8
function
MEP pathway of isoprenoid biosynthesis
product
1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase
essential
yes
ec
1.17.7.3
synonyms
ispG, yqfY
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0821 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,589,123 2,590,256
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H+ + H2O + oxidized [flavodoxin] --> 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin] (according to UniProt)
Protein family
ispG family (single member, according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
Structure
[PDB|3NOY] (from ''Aquifex aeolicus'', 51% identity) [Pubmed|20932974]
[AF|P54482]
[wiki|Localization]
secreted (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|144A8B3F5E534F3FD6EFC1E9A5ED650E09DCE885|spoIIIM]-[gene|4992F5C2ED7DE37C08D5715F7E8851F5FDB51059|ispG]
description
[Pubmed|12662922]
regulation
expressed early during sporulation in the mother cell ([protein|search|SigE], [wiki|SpoIIID]) [Pubmed|12662922,15383836]
regulatory mechanism
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
References
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Time of last update: 2024-12-16 10:44:28
Author of last update: Melvin.boenninger