qoxA
168
cytochrome aa3 quinol oxidase (subunit II)
locus
BSU_38170
Molecular weight
36.10 kDa
pI
4.8
function
respiration
product
cytochrome aa3 quinol oxidase (subunit II)
essential
no
ec
1.10.3.-
synonyms
qoxA, ipa-37d
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1622 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,917,296 3,918,261
Phenotypes of a mutant
inactivation of ''[gene|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]'' restores beta-lactam resistance in a ''[gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]'' mutant [Pubmed|22211522]
The protein
Catalyzed reaction/ biological activity
2 quinol + O2 --> 2 quinone + 2 H2O (according to UniProt)
Protein family
cytochrome c oxidase subunit 2 family (with [protein|72D5442C0EF3B404F7B615366E357B68C863CEB6|ctaC], according to UniProt)
Structure
[PDB|1FFT] ([protein|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]-[protein|CBF2DCFFD8195304F942ECBA8596444F0AC47CC6|qoxB]-[protein|CD4520EF427AEFDCF028AE063BC373FA6DCE379E|qoxC] complex from ''E. coli'', 35% identity) [Pubmed|11017202]
[AF|P34957]
[wiki|Localization]
cell membrane [Pubmed|18763711]
Additional information
the [protein|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]-[protein|CBF2DCFFD8195304F942ECBA8596444F0AC47CC6|qoxB]-[protein|CD4520EF427AEFDCF028AE063BC373FA6DCE379E|qoxC]-[protein|03335DC50CED7B38E2380640AE108ADA09A2CBDF|qoxD] cytochrome aa3 oxidase complex is sensitive to increased intracellular Mn2+ concentrations, this results in the generation of toxic reactive radicals [pubmed|34097790]
Expression and Regulation
Operons
genes
[gene|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]-[gene|CBF2DCFFD8195304F942ECBA8596444F0AC47CC6|qoxB]-[gene|CD4520EF427AEFDCF028AE063BC373FA6DCE379E|qoxC]-[gene|03335DC50CED7B38E2380640AE108ADA09A2CBDF|qoxD]
description
[Pubmed|1316894]
regulation
[protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
[protein|E15E9F88B0E03FE8834C646369D2F4B4E713EF41|citB]: translation control, [pubmed|], in [regulon|protein:E15E9F88B0E03FE8834C646369D2F4B4E713EF41|citB regulon]
additional information
the presence of an iron-responsive element bound by [wiki|CitB] between ''[wiki|feuA]'' and ''[wiki|feuB]'' suggests iron-dependent regulation by [wiki|CitB] [Pubmed|10468622]
Biological materials
Mutant
BKE38170 ([gene|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCATCCTTCCTATTGATG, downstream forward: _UP4_TGATATTGATCAGGAAAGGA
BKK38170 ([gene|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCATCCTTCCTATTGATG, downstream forward: _UP4_TGATATTGATCAGGAAAGGA
References
Reviews
Original Publications
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Time of last update: 2024-12-21 06:03:18
Author of last update: Jstuelk