tilS
168
trigger enzyme, tRNAIle-lysidine synthetase (modifies C34 at the wobble position of tRNA2Ile to lysidine) and part of a transcription activator
locus
BSU_00670
Molecular weight
53.30 kDa
pI
8.32
function
tRNA modification, control of [gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH] expression
product
tRNAIle-lysidine synthetase
essential
yes
ec
6.3.4.19
synonyms
tilS, yacA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0037 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
74,929 76,347
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
modifies C34 at the wobble position of tRNA2Ile to lysidine
ATP + cytidine34 in tRNAIle2 + L-lysine --> AMP + diphosphate + H+ + lysidine34 in tRNAIle2 (according to UniProt)
Protein family
tRNA(Ile)-lysidine synthase family (single member, according to UniProt)
[wiki|Domains]
The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding (according to UniProt)
Structure
[PDB|3A2K] (from ''Geobacillus kaustophilus'', complex with tRNA) [Pubmed|19847269]
[AF|P37563]
[wiki|Localization]
Cytoplasm (Homogeneous) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]-[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]-[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
description
[Pubmed|18179421]
regulation
induced by heat shock (class III)
regulatory mechanism
[protein|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]: activation, with [protein|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT] [Pubmed|24001521], in [regulon|protein:473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS regulon]
[protein|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]: activation, with [protein|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS] [Pubmed|24001521], in [regulon|protein:B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT regulon]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7608085], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
genes
[gene|7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A|spoIIE]-[gene|C8AA53889FC8B389E1D921B762BD78990C7A8E57|yabS]-[gene|E86A96B832351FF513DD9853EAD8998CC44C9951|yabT]-[gene|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]-[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]-[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
description
[pubmed|22383849]
regulation
expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]) [Pubmed|16497325]
sigma factors
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
Biological materials
Mutant
MGNA-B922 (yacA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1921 NBRP B. subtilis, Japan]
References
Reviews
Original Publications
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Time of last update: 2024-12-16 19:05:01
Author of last update: Melvin.boenninger