mgtE
168
primary magnesium transporter
locus
BSU_13300
Molecular weight
50.68 kDa
pI
4.61
function
magnesium uptake
product
primary magnesium transporter
essential
yes
synonyms
mgtE, ykoK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2239 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,396,013 1,397,368
Phenotypes of a mutant
essential [Pubmed|28189581]
a [gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE] mutant has a growth defect on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medium in the presence of citrate (due to the activity of [protein|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]) [Pubmed|24415722]
a [gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE] [gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ] mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medium in the presence of citrate (due to the activity of [protein|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]) [Pubmed|24415722]
The protein
Protein family
SLC41A transporter family (single member, according to UniProt)
[wiki|Domains]
2 [wiki|CBS domain]s (aa 140-203, aa 204-260) (according to UniProt)
Structure
[PDB|2YVX] (from ''Thermus thermophilus'', 34% identity) [Pubmed|17700703]
[PDB|2QBZ] (the mgtE riboswitch)
[AF|O34442]
Effectors of protein activity
activity is inhibited by high intracellular Mg2+ concentration [pubmed|19798051]
binds c-di-AMP [pubmed|31061098]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]
description
[pubmed|22383849]
regulation
induced at low magnesium concentration ([protein|C245476CE35028167510F67153F392D91447553F|M-box]) [Pubmed|17803910]
antitermination is modulated by [protein|43CFA3477B4EEB4CE0B2DF63ED2B5EDB171E79C7|nusG]-dependent transcription pausing just upstream of the [protein|C245476CE35028167510F67153F392D91447553F|M-box] [pubmed|39494516]
regulatory mechanism
M-box: RNA switch, Mg-responsive [protein|C245476CE35028167510F67153F392D91447553F|M-box], in [regulon|other_regulator:M-box|M-box]
additional information
MgtE stability is tightly regulated by the intramembrane protease [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH] (via the adaptor protein [protein|89C51789FBB2EE86771ABD8629FD99748ED5D4EF|yqgP]) [pubmed|31930742]
expression is increased as a result of loop duplication in [protein|F3DE42C41EF57DC5DFFF0D28129F2CE5DB781D99|L22] [pubmed|30853217]
Biological materials
Mutant
MGNA-B313 (mgtE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1312 NBRP B. subtilis, Japan]
GP2854 (''Δ''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]::''ermC''), available in [wiki|Jörg Stülke]'s lab
GP2762 (''Δ''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]::''spec''), available in [wiki|Jörg Stülke]'s lab
GP2763 (''Δ''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]::''kan Δ''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]::''spec''), available in [wiki|Jörg Stülke]'s lab
Expression vectors
pGP2927 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP2768 ''mgtE-3xFLAG spc'' (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
GP2869 (based on [wiki|pAC5]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-11-20 18:20:08
Author of last update: Jstuelk