mgtE

mgtE
168

primary magnesium transporter

locus
BSU_13300
Molecular weight
50.68 kDa
pI
4.61
Protein length
Gene length
function
magnesium uptake
product
primary magnesium transporter
essential
yes
synonyms
mgtE, ykoK

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2239 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,396,013  1,397,368
Phenotypes of a mutant
essential [Pubmed|28189581]
a [gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE] mutant has a growth defect on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medium in the presence of citrate (due to the activity of [protein|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]) [Pubmed|24415722]
a [gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE] [gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ] mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medium in the presence of citrate (due to the activity of [protein|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]) [Pubmed|24415722]
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The protein
Protein family
SLC41A transporter family (single member, according to UniProt)
[wiki|Domains]
2 [wiki|CBS domain]s (aa 140-203, aa 204-260) (according to UniProt)
Structure
[PDB|2YVX] (from ''Thermus thermophilus'', 34% identity) [Pubmed|17700703]
[PDB|2QBZ] (the mgtE riboswitch)
[AF|O34442]
Effectors of protein activity
activity is inhibited by high intracellular Mg2+ concentration [pubmed|19798051]
binds c-di-AMP [pubmed|31061098]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]
description
[pubmed|22383849]
regulation
induced at low magnesium concentration ([regulon|M-box|]) [Pubmed|17803910]
regulatory mechanism
M-box: RNA switch, Mg-responsive [protein|C245476CE35028167510F67153F392D91447553F|M-box], in [regulon|other_regulator:M-box|M-box]
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[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]

2024-06-08 11:56:43

Bzhu

88

e4e563f4357dec2c5b5815924274c7e7c86271e2

9D504E9849A9C48B5EBBF49732CD003FA8586A9A

additional information
MgtE stability is tightly regulated by the intramembrane protease [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH] (via the adaptor protein [protein|89C51789FBB2EE86771ABD8629FD99748ED5D4EF|yqgP]) [pubmed|31930742]
expression is increased as a result of loop duplication in [protein|F3DE42C41EF57DC5DFFF0D28129F2CE5DB781D99|L22] [pubmed|30853217]
Biological materials
Mutant
MGNA-B313 (mgtE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1312 NBRP B. subtilis, Japan]
GP2854 (''Δ''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]::''ermC''), available in [wiki|Jörg Stülke]'s lab
GP2762 (''Δ''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]::''spec''), available in [wiki|Jörg Stülke]'s lab
GP2763 (''Δ''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]::''kan Δ''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]::''spec''), available in [wiki|Jörg Stülke]'s lab
Expression vectors
pGP2927 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP2768 ''mgtE-3xFLAG spc'' (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
GP2869 (based on [wiki|pAC5]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
24079267,37948297
Original Publications
17803910,15096624,21315082,22970223,24415722,25182490,17700703,25367295,28189581,19798051,17671366,28747715,18832160,29967120,30853217,31061098,31930742,34472844,36515540,22383849

472E3A2407C83EEE26DB00079D185C4EA2988611

Page visits: 3960

Time of last update: 2024-06-22 16:04:39

Author of last update: Christoph.elfmann