zwf
168
glucose 6-phosphate dehydrogenase, pentose-phosphate pathway
locus
BSU_23850
Molecular weight
55.49 kDa
pI
5.28
function
initiation of the pentose phosphate pathway
product
glucose 6-phosphate dehydrogenase
essential
no
ec
1.1.1.49
synonyms
zwf, yqjJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0364 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,479,156 2,480,625
Phenotypes of a mutant
suppresses the growth defect of [gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR] mutants on gluconeogenic substrates [Pubmed|16272399]
a [gene|43CD2581DA421CABC66E30389640CC86E6B2D800|zwf] [gene|E3DAC5DE86EE0E34C6ECB6DE1E41309742465CF9|pgi] double mutant is unable to grow on minimal medium in the presence of [metabolite|glucose] (due to the accumulation of toxic [metabolite|glucose-1-phosphate]) [pubmed|4275311]
a [gene|43CD2581DA421CABC66E30389640CC86E6B2D800|zwf] [gene|E3DAC5DE86EE0E34C6ECB6DE1E41309742465CF9|pgi] [gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA] triple mutant is not viable [pubmed|30782637]
The protein
Catalyzed reaction/ biological activity
D-[metabolite|glucose-6-P] + [metabolite|NADP]+ --> [metabolite|D-glucono-1,5-lactone 6-P] + H+ + [metabolite|NADPH] (according to UniProt)
Protein family
glucose-6-phosphate dehydrogenase family (single member, according to UniProt)
[wiki|Cofactors]
Mg2+, Mn2+, Ca2+
Structure
[PDB|1DPG] (from ''Leuconostoc Mesenteroides'', 45% identity, 62% similarity) [Pubmed|7881907]
[AF|P54547]
Effectors of protein activity
[metabolite|NAD]+, [metabolite|NADP]+ and [metabolite|NADPH] affect the enzyme kinetic [Pubmed|6292660]
Additional information
The enzyme is a dimer [Pubmed|6292660]
Expression and Regulation
Operons
genes
[gene|43CD2581DA421CABC66E30389640CC86E6B2D800|zwf]
description
[Pubmed|12850135]
regulation
constitutive [Pubmed|12850135]
Biological materials
Mutant
MGNA-C392 (yqjJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2390 NBRP B. subtilis, Japan]
SM-NB3 (''zwf-spc''), available in [wiki|Anne Galinier]'s and [wiki|Boris Görke]'s labs [pubmed|16272399]
BKE23850 ([gene|43CD2581DA421CABC66E30389640CC86E6B2D800|zwf]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTAAAAGTACCTCACTTT, downstream forward: _UP4_TAATAAGAAGAAAAAAGCCG
BKK23850 ([gene|43CD2581DA421CABC66E30389640CC86E6B2D800|zwf]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTAAAAGTACCTCACTTT, downstream forward: _UP4_TAATAAGAAGAAAAAAGCCG
Expression vectors
pGP2887: expression of Strep-''zwf'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP1401 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
References
**Reviews
Original Publications
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Time of last update: 2024-12-20 20:18:28
Author of last update: Jstuelk