ytxH
168
general stress protein
locus
BSU_29770
Molecular weight
16.54 kDa
pI
5.11
function
unknown
product
unknown
essential
no
ec
null
synonyms
ytxH, csb40
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4980 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,047,107 → 3,047,562
The protein
Structure
[AF|P40780]
Modification
phosphorylated on Arg-50 [Pubmed|22517742]
phosphorylated on Arg-151 [pubmed|31221751]
Expression and Regulation
Operons
genes
[gene|00A42FDB559BE2647CD682180420A435E2E7035F|facZ]-[gene|43BF293F735ED783D36F33AA4701D57F2AED6657|ytxH]-[gene|8B4E1D087B75B5E77BCB60FF3465270CEB72B028|brxC]
description
[Pubmed|8733232]
regulation
induced by stress ([protein|search|SigB]) [Pubmed|8733232,11544224]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|8733232,11544224], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|8733232], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
Biological materials
Mutant
GP3769 (Δ[gene|43BF293F735ED783D36F33AA4701D57F2AED6657|ytxH]::cat) available in [wiki|Jörg Stülke]'s lab
GP3770 (Δ[gene|6EF9B43AFDE3AEBE0DA5EDCCEDA99331BA01AE5D|yhaH]::kan Δ[gene|43BF293F735ED783D36F33AA4701D57F2AED6657|ytxH]::cat) available in [wiki|Jörg Stülke]'s lab
MGNA-B528 (ytxH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1527 NBRP B. subtilis, Japan]
BKE29770 (Δ[gene|43BF293F735ED783D36F33AA4701D57F2AED6657|ytxH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATTATTCCTCCTCTTA, downstream forward: _UP4_TGAGCAGGGAAGGAAGAGCT
BKK29770 (Δ[gene|43BF293F735ED783D36F33AA4701D57F2AED6657|ytxH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATTATTCCTCCTCTTA, downstream forward: _UP4_TGAGCAGGGAAGGAAGAGCT
Expression vectors
pGP3642: expression of [gene|43BF293F735ED783D36F33AA4701D57F2AED6657|ytxH] by [wiki|pBQ200] in B. subtilis, available in [wiki|Jörg Stülke]'s lab
pGP3655 (N-terminal Strep-tag, purification from E. coli, in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
pGP3671 (N-terminal His-tag, purification from E. coli, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-11-09 23:26:21
Author of last update: Jstuelk