mprF
168
synthesis of lysylphosphatidylglycerol (L-PG), lysinylation of phosphatidylglycerol provides some resistance against positively charged antimicrobial peptides
locus
BSU_08425
Molecular weight
96.00 kDa
pI
9
function
lysyl-phosphatidylglycerol lipid biosynthesis
product
lysyl-phosphatidylglycerol synthase
essential
no
synonyms
mprF, yfiW, yfiX
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2898 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
916,778 919,348
Phenotypes of a mutant
reduced conjugation of ICEBs1 [Pubmed|25069588,26833415]
overexpression results in increased conjugation of ICEBs1 [Pubmed|26833415]
suppression of the growth defect of a strain lacking all four class A [wiki|penicillin-binding proteins] ([gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] [gene|E50D4B30C2A0987A356C988AF8A4951C0CDF6C06|pbpD] [gene|73275060537497E6A9859856C9F040763E36316B|pbpF] [gene|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|pbpG]) on complex medium [pubmed|36744964]
The protein
Catalyzed reaction/ biological activity
1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + L-lysyl-tRNALys --> 1,2-diacyl-sn-glycero-3-phospho-1ʼ-(3ʼ-O-L-lysyl)-sn-glycerol + tRNALys (according to UniProt)
Protein family
LPG synthase family (single member, according to UniProt)
Structure
[PDB|4V36] (Lys-tRNA(Lys)-dependent lysyl-phosphatidylglycerol synthase domain from ''B. licheniformis'', 76% identity) [Pubmed|26261323]
[AF|C0H3X7]
[wiki|Localization]
cell membrane at the septum [Pubmed|15743965]
Expression and Regulation
Operons
genes
[gene|4385EA135BDEB54B33C9A8E39150A60C83C0CEEA|mprF]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-C310 (yfiX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2308 NBRP B. subtilis, Japan]
GP2246 (''[gene|4385EA135BDEB54B33C9A8E39150A60C83C0CEEA|mprF]''::''ermC''), available in [wiki|Jörg Stülke]'s lab
BKE08425 ([gene|4385EA135BDEB54B33C9A8E39150A60C83C0CEEA|mprF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08425 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGGTCTCTCCAATCATA, downstream forward: _UP4_GTCTAATAAGACGGAGTCTT
BKK08425 ([gene|4385EA135BDEB54B33C9A8E39150A60C83C0CEEA|mprF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08425 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGGTCTCTCCAATCATA, downstream forward: _UP4_GTCTAATAAGACGGAGTCTT
References
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Time of last update: 2024-12-22 08:46:51
Author of last update: Jstuelk