mtnW
168
2,3-diketo-5-methylthiopentyl-1-phosphate enolase, Rubisco-like protein
locus
BSU_13590
Molecular weight
44.89 kDa
pI
5.28
function
methionine salvage
product
2,3-diketo-5-methylthiopentyl-1-phosphate enolase
essential
no
ec
null
synonyms
mtnW, ykrW
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1850 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,427,034 1,428,278
The protein
Catalyzed reaction/ biological activity
5-methylsulfanyl-2,3-dioxopentyl phosphate --> 2-hydroxy-5-methylsulfanyl-3-oxopent-1-enyl phosphate (according to UniProt)
Protein family
RuBisCO large chain family (single member, according to UniProt)
Structure
[PDB|2ZVI] [Pubmed|19690372]
[AF|O31666]
Expression and Regulation
Operons
genes
[gene|42D2F9B958F62DFE447A1321E4109D676ECB899C|mtnW]-[gene|91633E6AF9A715166AE05BD7E540463DE352AA20|mtnX]-[gene|337F1E27C2E380DF8DCADE3581F74CFF52AFD224|mtnB]-[gene|C7D9E701B2CA2C9E85D69CAD6E0A45BE590BAED9|mtnD]
description
[Pubmed|12022921]
regulation
induced by methionine starvation ([wiki|S-box]) [Pubmed|10094622]
regulatory mechanism
S-box: RNA switch, , the [wiki|S-box] [wiki|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|other_regulator:S-box|S-box]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12022921], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE13590 ([gene|42D2F9B958F62DFE447A1321E4109D676ECB899C|mtnW]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCAGAGCTCCTTTCAT, downstream forward: _UP4_AAATGGGGAAAGGCTGAAGC
BKK13590 ([gene|42D2F9B958F62DFE447A1321E4109D676ECB899C|mtnW]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCAGAGCTCCTTTCAT, downstream forward: _UP4_AAATGGGGAAAGGCTGAAGC
References
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Time of last update: 2025-03-17 11:50:12
Author of last update: Melvin.boenninger