comGA
168
late competence protein, traffic ATPase, binds and transports transforming DNA, required for growth arrest of competent cells
locus
BSU_24730
Molecular weight
40.30 kDa
pI
8.89
function
genetic competence
product
traffic ATPase
essential
no
synonyms
comGA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2804 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,559,007 2,560,077
Phenotypes of a mutant
loss of [wiki|genetic competence]
The protein
Catalyzed reaction/ biological activity
binds and transports transforming DNA
sequesters [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] during expression of [Pubmed|genetic competence] to inhibit cell elongation during escape from competence [Pubmed|26091431]
Protein family
GSP E family (single member, according to UniProt)
Structure
[PDB|4PHT] (from Vibrio vulnificus, 38% identity) [pubmed|25092625]
[AF|P25953]
Modification
phosphorylated on Arg-28, Arg-71- and Arg-330 [Pubmed|22517742]
[wiki|Localization]
peripheral membrane protein [Pubmed|9723928]
localizes to cell poles in competent cells [Pubmed|16009133]
localizes to one cell pole, this depends on [protein|954359717E15DFC106A9D8111C6637E46B16F8C9|comEB] [Pubmed|31954084,21278288]
Additional information
ComGA stability depends on [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|21564336]
Expression and Regulation
Operons
genes
[gene|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|comGA]-[gene|EA1C841FC77A1994A85E2E0C0A554482C3EC9323|comGB]-[gene|19D76B80386F825109C5FB4A34881D5D20F366DB|comGC]-[gene|D323AEF5AFB4FD2A074ABAB76A39BE26A4C6436F|comGD]-[gene|FF4DB7FDCEB440064E11E65CB0DBFB93FC785CF0|comGE]-[gene|DD266DC0D78B5C5E82EED7CC7F55D0CF8FB4BF7A|comGF]-[gene|89442BFDFBFF264D0C7F12DEA6F8130C55D1F3F8|comGG]-[gene|8F3751A3D0910C9BED7FBCE90AFF7FDACFDCBC0B|spoIIIL]
description
[Pubmed|26735940,2507524]
regulation
expressed early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|26735940]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|8196543], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2507524], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|26735940], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
additional information
the ''[wiki|comGA]-[wiki|comGB]-[wiki|comGC]-[wiki|comGD]-[wiki|comGE]-[wiki|comGF]-[wiki|comGG]-[protein|search|spoIIIL]'' operon requires [wiki|NusA] for expression [http://www.nature.com/articles/nmicrobiol20157 Reference]
Biological materials
Mutant
BKE24730 ([gene|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|comGA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTGATTCCCTCTCCTT, downstream forward: _UP4_TAGAAAAGTCTGGTTGTTAA
BKK24730 ([gene|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|comGA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTGATTCCCTCTCCTT, downstream forward: _UP4_TAGAAAAGTCTGGTTGTTAA
BP914 (([gene|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|comGA]-[gene|EA1C841FC77A1994A85E2E0C0A554482C3EC9323|comGB]-[gene|19D76B80386F825109C5FB4A34881D5D20F366DB|comGC]-[gene|D323AEF5AFB4FD2A074ABAB76A39BE26A4C6436F|comGD]-[gene|FF4DB7FDCEB440064E11E65CB0DBFB93FC785CF0|comGE]-[gene|DD266DC0D78B5C5E82EED7CC7F55D0CF8FB4BF7A|comGF]-[gene|89442BFDFBFF264D0C7F12DEA6F8130C55D1F3F8|comGG])::phleo), available in [wiki|Fabian Commichau]'s lab
GFP fusion
GP1640 ''trpC2'' ''[gene|154862E3FFA7CEBA5725BAD08E09D0E1C6B7F946|ymdB]''::''spc'' P''comG-gfp'' (''cat''), available in [wiki|Jörg Stülke]'s lab
GP1643 P''comG-gfp'' (''cat'') in the NCIB3610 background, available in [wiki|Jörg Stülke]'s lab
PG389 [gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]::(p[gene|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|comGA]-lacZ gfp cat) [pubmed|26110430], available in [wiki|Leendert Hamoen]'s and [wiki|Jörg Stülke]'s labs
References
Reviews
Original Publications
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Time of last update: 2024-10-04 15:56:54
Author of last update: Jstuelk