ltaS
168
major polyglycerolphosphate lipoteichoic acid synthase, required for proper colony development
locus
BSU_07710
Molecular weight
74.05 kDa
pI
5.2
function
biosynthesis of lipoteichoic acid
product
major lipoteichoic acid synthase
essential
no
ec
2.7.8.-
synonyms
ltaS, yflE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1368 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
842,047 843,996
Phenotypes of a mutant
induction of [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM] and [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX] activities [Pubmed|23103977]
reduced [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]-dependent expression of ''[gene|E8B88CBE4F9121DFEEF099D7947CD1E1AE656160|lytF]'' [Pubmed|25288647]
extended lag phase [pubmed|29114240]
reduced colony size with accumulation of dead cells in the colonies [pubmed|29114240]
suppression of the growth and morphology defects of a [gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR] mutant [pubmed|30478337]
poor growth of [gene|418900B6DBDF3EF300C930B0C75E31B939F3CE00|ltaS] [gene|8BF7EB539B53D75BD30BD30ABF27106D6A0B030B|ftsE] and [gene|418900B6DBDF3EF300C930B0C75E31B939F3CE00|ltaS] [gene|E22D0F0AC56DC95AFC02E7F55FCD5FAA44B6B490|ftsX] double mutants [pubmed|39185233]
poor growth of a [gene|418900B6DBDF3EF300C930B0C75E31B939F3CE00|ltaS] [gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] double mutant [pubmed|39185233]
inactivation suppresses the essentiality of [gene|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl] [pubmed|39185233]
The protein
Protein family
[wiki|LTA synthase family] (according to UniProt)
[wiki|Cofactors]
Mn(2+) [pubmed|37794032]
Structure
[PDB|2W8D] [pubmed|19229300]
[AF|O34952]
Modification
phosphorylated on Thr-297 [Pubmed|19229300]
can be phosphorylated by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC] in vitro [pubmed|30478337]
Paralogous protein(s)
[protein|880C655DC7149F6EDC21BD2CF8F9F2C9FBEB2CDB|yvgJ], [protein|CCD06F344E3C87E77C3D13883CE2B927F372A84E|yfnI], [protein|2B33F2F9C2FB0767B72A29D060037393C88ACEE5|yqgS]
[wiki|Localization]
cell membrane, at the septum of the cell [Pubmed|15743965]
extracellular (signal peptide) [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|418900B6DBDF3EF300C930B0C75E31B939F3CE00|ltaS]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-C256 (yflE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2254 NBRP B. subtilis, Japan]
GP1389 ''ltaS::aphA3'', available in [wiki|Jörg Stülke]'s lab
GP1396 ''ltaS::tet'', available in [wiki|Jörg Stülke]'s lab
BKE07710 ([gene|418900B6DBDF3EF300C930B0C75E31B939F3CE00|ltaS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTACACTCCTTTTTT, downstream forward: _UP4_TAAGAAAAAGCGGAGAGGTT
BKK07710 ([gene|418900B6DBDF3EF300C930B0C75E31B939F3CE00|ltaS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTACACTCCTTTTTT, downstream forward: _UP4_TAAGAAAAAGCGGAGAGGTT
References
Reviews
Original Publications
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Time of last update: 2024-12-29 11:46:52
Author of last update: Jstuelk