fabHA
168
beta-ketoacyl-acyl carrier protein synthase III, principal condensing enzyme responsible for the initiation of fatty acid synthesis in non-stressed B. subtilis cells
locus
BSU_11330
Molecular weight
33.58 kDa
pI
5.05
function
fatty acid biosynthesis
product
beta-ketoacyl-acyl carrier protein synthase III
essential
no
ec
2.3.1.180
synonyms
fabHA, yjaX
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0332 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,208,222 1,209,160
Phenotypes of a mutant
significant increase in the proportion of straight-chain fatty acids with a concomitant increase in 31:0-carbon phosphatidylethanolamine species [Pubmed|21542858]
The protein
Catalyzed reaction/ biological activity
acetyl-CoA + H+ + malonyl-[ACP] --> 3-oxobutanoyl-[ACP] + CO2 + CoA (according to UniProt)
Protein family
[wiki|thiolase-like superfamily] (according to UniProt)
Structure
[PDB|8VD9] [Pubmed|38310992]
[PDB|8VDA] (complex with CoA) [Pubmed|38310992]
[AF|O34746]
Paralogous protein(s)
[protein|FC818B3A8A3080231C1F8DE95377325C8E754DCE|fabHB], one of the two proteins has to be present for viability [Pubmed|17114254]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
affinity for butyryl-CoA, but prefers acetyl-CoA in fatty acid biosynthesis [Pubmed|19820084]
Expression and Regulation
Operons
genes
[gene|40E064365588CFED483FBFED02BFC0DA531CCC4A|fabHA]-[gene|5D5D9A544295DD9B55C75A8CF47AB19935E740C6|fabF]
description
[Pubmed|12737802]
regulation
''[wiki|fabHA]'': expressed when the cells experience a lack of malonyl-CoA ([protein|search|FapR]) [Pubmed|12737802]
regulatory mechanism
[protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]: repression, [Pubmed|12737802], in [regulon|protein:FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12737802], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|21542858], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]
Biological materials
Mutant
MGNA-B145 (yjaX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1144 NBRP B. subtilis, Japan]
BKE11330 ([gene|40E064365588CFED483FBFED02BFC0DA531CCC4A|fabHA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAGGGAAGACTCCTTTA, downstream forward: _UP4_TAAAAAAAGGTGAGGTGCAC
BKK11330 ([gene|40E064365588CFED483FBFED02BFC0DA531CCC4A|fabHA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAGGGAAGACTCCTTTA, downstream forward: _UP4_TAAAAAAAGGTGAGGTGCAC
References
Reviews
Original Publications
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Time of last update: 2024-12-30 17:22:11
Author of last update: Jstuelk