nasB
168
nitrate reductase (electron transfer subunit)
locus
BSU_03320
Molecular weight
84.57 kDa
pI
5.48
function
utilization of nitrate
product
nitrate reductase (electron transfer subunit)
essential
no
synonyms
nasB, nasBA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1251 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
360,442 362,757
The protein
[wiki|Cofactors]
FAD [Pubmed|11289299]
Structure
[PDB|3KLJ] (from Clostridium acetobutylicum, corresponds to aa 2 ... 371, 27% identity) [pubmed|20017214]
[AF|P42433]
Paralogous protein(s)
[protein|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]
Expression and Regulation
Operons
genes
[gene|3F87227DC3371746B3823730B6A7024D348C151E|nasB]-[gene|EC8BF9A3BA88FE2A78C6F78A888E0D1E25C47BC5|nasC]-[gene|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]-[gene|A8998F46F68155A5C8AE2A2FBB95CA5A1635BB2B|nasE]-[gene|159A6D0D45B5F0F055228357FD9A3BF8E5A999E5|nasF]
description
[Pubmed|7836289]
regulation
''[protein|search|nasD]'': expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]
regulatory mechanism
[protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]: activation, [Pubmed|10972836], in [regulon|protein:1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD regulon]
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [PubMed|8799114,9765565], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|EC6697D5D945B7E5083AFED9218748763C443278|nsrR]: repression, [Pubmed|16885456], in [regulon|protein:EC6697D5D945B7E5083AFED9218748763C443278|nsrR regulon]
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7836289], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
1A971 ( ''nasB''::''erm''), [Pubmed|7868621], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A971&Search=1A971 BGSC]
BKE03320 ([gene|3F87227DC3371746B3823730B6A7024D348C151E|nasB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTATCAGACCTCCTTTG, downstream forward: _UP4_TGAGGCATTTTGACTGAACG
BKK03320 ([gene|3F87227DC3371746B3823730B6A7024D348C151E|nasB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTATCAGACCTCCTTTG, downstream forward: _UP4_TGAGGCATTTTGACTGAACG
References
Reviews
Original Publications
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Time of last update: 2024-11-08 08:44:22
Author of last update: Jstuelk