opuBA

opuBA
168

choline and arsenocholine ABC transporter (ATP-binding protein)

Locus
BSU_33730
Molecular weight
42.92 kDa
Isoelectric point
5.65
Protein length
381 aaSequenceBlast
Gene length
1146 bpSequenceBlast
Function
compatible solute transport
Product
choline and arsenocholine ABC transporter (ATP-binding protein)
Essential
no
Synonyms
opuBA, proV
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG1125 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, GbsR regulon, OpcR regulon, RemA regulon

Gene
Coordinates
3,462,105  3,463,250
The protein
Catalyzed reaction/ biological activity
uptake of choline and arsenocholine PubMed
Protein family
ABC transporter superfamily (according to UniProt)
Domains
2 CBS domains (aa 256-314, aa 316-374) (according to UniProt)
ABC transporter domain (aa 2-236) (according to UniProt)
Structure
2IT1 (PDB) (from Pyrococcus horikoshii, 43% identity)
Q45460 (AlphaFold)
Paralogous protein(s)
MsmX, OpuCA, OpuAA
Localization
associated to the membrane (via OpuBB-OpuBD) PubMed
Expression and Regulation
Operons
Genes
opuBA-opuBB-opuBC-opuBD
Description
PubMed
Regulation
induced by choline (GbsR) PubMed
Regulatory mechanism
GbsR: repression, (transcriptional roadblock) PubMed, in gbsR regulon
OpcR: repression, (sterical interference with RNA polymerase access) PubMed, in opcR regulon
RemA: activation, PubMed, in remA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
An antisense RNA is predicted for'opuBD' PubMed
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opuBA→opuBD

2025-04-03 13:33:40

ghost

91

f5d474616565c5e32550f1c34aef09f972669a8e

1F7D81939583251B3B9B9F3D0D915C77CF89D428

Biological materials
Mutant
BKE33730 (opuBA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAACATCGCACCTCCAAAA,  downstream forward: _UP4_TAGGGAGGGGCGATAAGTCA
BKK33730 (opuBA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAACATCGCACCTCCAAAA,  downstream forward: _UP4_TAGGGAGGGGCGATAAGTCA
Expression vectors
pGP2938: (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
pGP3013: (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
LacZ fusion
pGP3880 (kan, based on pAC7]), available in Jörg Stülke's lab
GP4303 (opuBA-lacZ::aphA3), available in Jörg Stülke's lab
References
Reviews
Loading
Original Publications
Mahendran A, Orlando BJGenome wide structural prediction of ABC transporter systems in Bacillus subtilis.Frontiers in microbiology. 2024; 15:1469915. PMID: 39397791
Warmbold B, Ronzheimer S, Freibert SA, Seubert A, Hoffmann T, Bremer ETwo MarR-Type Repressors Balance Precursor Uptake and Glycine Betaine Synthesis in Bacillus subtilis to Provide Cytoprotection Against Sustained Osmotic Stress.Frontiers in microbiology. 2020; 11:1700. PMID: 32849357
Rath H, Reder A, Hoffmann T, Hammer E, Seubert A, Bremer E, Völker U, Mäder U Management of Osmoprotectant Uptake Hierarchy in via a SigB-Dependent Antisense RNA. Frontiers in microbiology. 2020; 11:622. doi:10.3389/fmicb.2020.00622. PMID:32373088
Hoffmann T, Warmbold B, Smits SHJ, Tschapek B, Ronzheimer S, Bashir A, Chen C, Rolbetzki A, Pittelkow M, Jebbar M, Seubert A, Schmitt L, Bremer E Arsenobetaine: an ecophysiologically important organoarsenical confers cytoprotection against osmotic stress and growth temperature extremes. Environmental microbiology. 2017 Nov 21; . doi:10.1111/1462-2920.13999. PMID:29159878
Lee CH, Wu TY, Shaw GC Involvement of OpcR, a GbsR-type transcriptional regulator, in negative regulation of two evolutionarily closely related choline uptake genes in Bacillus subtilis. Microbiology (Reading, England). 2013 Oct; 159(Pt 10):2087-96. doi:10.1099/mic.0.067074-0. PMID:23960087
Winkelman JT, Bree AC, Bate AR, Eichenberger P, Gourse RL, Kearns DB RemA is a DNA-binding protein that activates biofilm matrix gene expression in Bacillus subtilis. Molecular microbiology. 2013 Jun; 88(5):984-97. doi:10.1111/mmi.12235. PMID:23646920
Nau-Wagner G, Opper D, Rolbetzki A, Boch J, Kempf B, Hoffmann T, Bremer E Genetic control of osmoadaptive glycine betaine synthesis in Bacillus subtilis through the choline-sensing and glycine betaine-responsive GbsR repressor. Journal of bacteriology. 2012 May; 194(10):2703-14. doi:10.1128/JB.06642-11. PMID:22408163
Hoffmann T, Bremer E Protection of Bacillus subtilis against cold stress via compatible-solute acquisition. Journal of bacteriology. 2011 Apr; 193(7):1552-62. doi:10.1128/JB.01319-10. PMID:21296969
Kappes RM, Kempf B, Kneip S, Boch J, Gade J, Meier-Wagner J, Bremer E Two evolutionarily closely related ABC transporters mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine in Bacillus subtilis. Molecular microbiology. 1999 Apr; 32(1):203-16. . PMID:10216873
Quentin Y, Fichant G, Denizot F Inventory, assembly and analysis of Bacillus subtilis ABC transport systems. Journal of molecular biology. 1999 Apr 02; 287(3):467-84. . PMID:10092453
Nau-Wagner G, Boch J, Le Good JA , Bremer E High-affinity transport of choline-O-sulfate and its use as a compatible solute in Bacillus subtilis. Applied and environmental microbiology. 1999 Feb; 65(2):560-8. . PMID:9925583

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Time of last update: 2025-04-08 05:02:49

Author of last update: Jstuelk