opuBA
168
choline and arsenocholine [wiki|ABC transporter] (ATP-binding protein)
locus
BSU_33730
Molecular weight
42.92 kDa
pI
5.65
function
compatible solute transport
product
choline and arsenocholine [wiki|ABC transporter] (ATP-binding protein)
essential
no
synonyms
opuBA, proV
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1125 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,462,105 3,463,250
The protein
Catalyzed reaction/ biological activity
uptake of choline and arsenocholine [pubmed|29159878]
Protein family
[wiki|ABC transporter superfamily] (according to UniProt)
[wiki|Domains]
2 [wiki|CBS domain]s (aa 256-314, aa 316-374) (according to UniProt)
[wiki|ABC transporter domain] (aa 2-236) (according to UniProt)
Structure
[PDB|2IT1] (from Pyrococcus horikoshii, 43% identity)
[AF|Q45460]
Paralogous protein(s)
[protein|434F6C546F783503A82D390FD238F05025FC1802|msmX], [protein|75DB6231129C28C5D3791BA43A1261CD46A861E8|opuCA], [protein|FB2220730013A98D7C979062A4A451518B92DF09|opuAA]
[wiki|Localization]
associated to the membrane (via [protein|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|opuBB]-[protein|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]) [Pubmed|10092453]
Expression and Regulation
Operons
genes
[gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]-[gene|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|opuBB]-[gene|C48798FE13B1521236F5BC5786B9A02D7BC9A336|opuBC]-[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]
description
[Pubmed|10216873]
regulation
induced by choline ([protein|search|GbsR]) [Pubmed|22408163]
regulatory mechanism
[protein|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|gbsR]: repression, (transcriptional roadblock) [Pubmed|32849357,22408163], in [regulon|protein:5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|gbsR regulon]
[protein|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|opcR]: repression, (sterical interference with RNA polymerase access) [Pubmed|32849357,23960087], in [regulon|protein:9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|opcR regulon]
[protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]: activation, [Pubmed|23646920], in [regulon|protein:6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10216873], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
An antisense RNA is predicted for'[protein|search|opuBD]' [PubMed|20525796]
Biological materials
Mutant
BKE33730 ([gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACATCGCACCTCCAAAA, downstream forward: _UP4_TAGGGAGGGGCGATAAGTCA
BKK33730 ([gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACATCGCACCTCCAAAA, downstream forward: _UP4_TAGGGAGGGGCGATAAGTCA
Expression vectors
pGP2938: (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP3013: (N-terminal Strep-tag, purification from ''E. coli'', in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP3880 (kan, based on [wiki|pAC7]]), available in [wiki|Jörg Stülke]'s lab
GP4303 ([gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]-lacZ::aphA3), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-30 12:03:23
Author of last update: Jstuelk