proJ
168
glutamate 5-kinase
locus
BSU_18470
Molecular weight
40.20 kDa
pI
5.3
function
osmoadaptive de novo production of proline
product
glutamate 5-kinase
essential
no
ec
2.7.2.11
synonyms
proJ, yohA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0263 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,015,733 2,016,848
The protein
Catalyzed reaction/ biological activity
ATP + L-glutamate --> ADP + L-glutamyl 5-phosphate (according to UniProt)
Protein family
glutamate 5-kinase family (with [protein|5592C5163CF024289B850916516E2C8C7AD43136|proB], according to UniProt)
[wiki|Domains]
PUA domain (aa 280-356) (according to UniProt)
Structure
[PDB|4Q1T] (from ''Burkholderia thailandensis'', 39% identity)
[AF|O07509]
Paralogous protein(s)
[protein|5592C5163CF024289B850916516E2C8C7AD43136|proB]
Expression and Regulation
Operons
genes
[gene|542871C77BE841AA59A65FFE8A44AB85BF9180F0|proH]-[gene|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ]
description
[Pubmed|21296969]
regulation
expressed under conditions of osmotic stress [Pubmed|35593133,21784929]
induced upon antibiotic stress [pubmed|35593133]
expression
expression is heterogeneously activated [pubmed|35593133]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21784929], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP817 (spc) available in [wiki|Jörg Stülke]'s lab
1A635 ( ''proJ''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A635&Search=1A635 BGSC]
BKE18470 ([gene|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTTTTCATGCTTGTATCAG, downstream forward: _UP4_TAATTTGCAGAAAAAAACCC
BKK18470 ([gene|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTTTTCATGCTTGTATCAG, downstream forward: _UP4_TAATTTGCAGAAAAAAACCC
References
Reviews
Original Publications
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Time of last update: 2025-04-03 06:34:49
Author of last update: Jstuelk