mrfB
168
Mg2+-dependent 3'-5' DNA exonuclease, part of a novel nucleotide excision repair pathway (with [protein|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA])
locus
BSU_22210
Molecular weight
47.83 kDa
pI
7.16
function
mitomycin C-specific DNA damage repair
product
metal-dependent DNA exonuclease
essential
no
synonyms
mrfB, yprB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3359 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,333,324 2,334,565
Phenotypes of a mutant
sensitive to mitomycin C [pubmed|30379365]
The protein
Catalyzed reaction/ biological activity
repair of mitomycin C-induced DNA adducts [pubmed|38661211]
[wiki|Cofactors]
Mg2+ [pubmed|30379365]
Structure
[PDB|8UN9] [pubmed|38661211]
[AF|P50837]
Expression and Regulation
Operons
genes
[gene|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA]-[gene|3F1D78CAAA4B5057DEBB24C4D382F96DA4AA4852|mrfB]-[gene|FA8D9AA0968365522DEFC05B277123FC96482044|cotD]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A413 (yprB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/413 NBRP B. subtilis, Japan]
BKE22210 ([gene|3F1D78CAAA4B5057DEBB24C4D382F96DA4AA4852|mrfB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATGACATATCCGGCCTCC, downstream forward: _UP4_TAAATATTCCCCGGGAAAGC
BKK22210 ([gene|3F1D78CAAA4B5057DEBB24C4D382F96DA4AA4852|mrfB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATGACATATCCGGCCTCC, downstream forward: _UP4_TAAATATTCCCCGGGAAAGC
References
Reviews
Research papers
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Time of last update: 2025-04-11 19:43:33
Author of last update: Jstuelk