rnjA
168
RNase J1, important for the degradation of RNA fragments containing transcription terminators, resolves stalled transcription complexes
locus
BSU_14530
Molecular weight
61.34 kDa
pI
5.9
function
RNA processing
product
RNase J1
essential
no
synonyms
rnjA, ykqC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0595 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,523,118 1,524,785
Phenotypes of a mutant
growth is strongly impaired (doubling time in complex medium 145 minutes vs. 43 minutes for the wild type) [pubmed|26883633]
the mutant is strongly impaired in [wiki|sporulation] [Pubmed|23504012]
loss of [wiki|genetic competence] [pubmed|33897624]
it is not possible to construct a ''[gene|3EB289D0F58A58C693AB588798EE66A731341999|rnjA] [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|rny]'' double mutant [Pubmed|23504012]
The protein
Catalyzed reaction/ biological activity
endonuclease and 5'-3' exonuclease
degrades [protein|8950A485A0FEA4BA2FFC0C74A8D3D5D73D44B3FF|SR5] and ''[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]'' RNAs [pubmed|27449348]
degrades [gene|search|yflS ]mRNA [pubmed|28436820]
resolves stalled transcription complexes by a “torpedo” mechanism, whereby RNase J1 degrades the nascent RNA and causes the transcription complex to disassemble upon collision with [wiki|RNA polymerase] [pubmed|31840842]
maturation of [gene|A0E4AC7E74B5374198D047589629DA96A681CAF4|bsrA] and [gene|E85341621B3835F63F07C506E08B73A92E588B1F|bsrB] RNAs [pubmed|37369528]
Protein family
[wiki|metallo-beta-lactamase superfamily] (according to UniProt)
Structure
[PDB|3ZQ4] [Pubmed|21893285]
[AF|Q45493]
Effectors of protein activity
initeraction with [protein|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]-[protein|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1] stimulates activity [Pubmed|27449348]
a NAD-cap stabilizes RNA against exonucleolytic attack by RNase J1 [pubmed|30110644]
Paralogous protein(s)
[protein|B73CE26E7AEFACFA82C67F3E7DBBB3F766F739CA|rnjB]
[wiki|Localization]
cytoplasm, colocalizes with the ribosomes [Pubmed|27708634]
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
required for [gene|3E1131CFA3EDEB3865638F09BE2F6B6ED2570BE2|thrS] RNA processing, involved in maturation of the 5-end of the 16S rRNA
Expression and Regulation
Operons
genes
[gene|5F41273FB867C688011738CD45AA6FF5D3987EFE|rpoY]-[gene|3EB289D0F58A58C693AB588798EE66A731341999|rnjA]
description
[Pubmed|24187087]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|24187087], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP41 (''rnjA'' under control of p(xyl)), available in [wiki|Jörg Stülke]'s lab
SSB342 (''rnjA'' under pspac), cat, available in [wiki|Harald Putzer]'s lab
GP2506 (Δ[gene|3EB289D0F58A58C693AB588798EE66A731341999|rnjA]::spc) available in [wiki|Jörg Stülke]'s lab [pubmed|33897624]
Expression vectors
for chromosomal expression of RNase J1-Strep (spc): GP1034, available in [wiki|Jörg Stülke]'s lab
for chromosomal expression of RNase J1-Strep (cat): GP1042, available in [wiki|Jörg Stülke]'s lab
pGP1441: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab, [pubmed|19193632]
FLAG-tag construct
GP1020 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
GP1075 (aphA3), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP418 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
GFP fusion
GP1694 (in pHJS-105 [Pubmed|26110430]), expression of '' rnjA-sfGFP''::''spc'' under a xylose-inducible promoter in '' B. subtilis'', available in [wiki|Jörg Stülke]'s lab [Pubmed|27708634]
GP1722 (in [wiki|pBP43]), expression of '' rnjA-GFP''::''spc'' under the native promoter, available in [wiki|Jörg Stülke]'s lab [Pubmed|27708634]
labs
[wiki|Harald Putzer], IBPC Paris, France [http://www.ibpc.fr/UPR9073/putzer/recherches_harald.htm Homepage]
[wiki|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]
[wiki|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-20 12:41:47
Author of last update: Jstuelk