pta
168
phosphotransacetylase
locus
BSU_37660
Molecular weight
34.64 kDa
pI
4.65
function
overflow metabolism
product
phosphotransacetylase
essential
no
ec
2.3.1.8
synonyms
pta, ipa-88d, ywfJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0280 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,865,355 3,866,326
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
The protein
Catalyzed reaction/ biological activity
acetyl-CoA + phosphate --> acetyl phosphate + CoA (according to UniProt)
Protein family
phosphate acetyltransferase and butyryltransferase family (with [protein|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb], according to UniProt)
Structure
[PDB|1XCO] (complex with acetylphosphate)[pubmed|16283428]
[PDB|1TD9]
[AF|P39646]
Modification
phosphorylation on (Ser-123 OR Thr-128 OR Ser-129) [Pubmed|17218307]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|3E343C05EE6FF04D5A54066E19DC6576784E09E7|pta]
description
[Pubmed|10423526]
regulation
expression activated by glucose (2.6 fold) (CcpA) [Pubmed|12850135,10559153]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: activation, [Pubmed|12850135,10559153], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10423526], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP668 (aphA3), available in [wiki|Jörg Stülke]'s lab
BKE37660 ([gene|3E343C05EE6FF04D5A54066E19DC6576784E09E7|pta]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAATAAAACCTCCTCAAA, downstream forward: _UP4_TAATAAAATTGAAGACAATG
BKK37660 ([gene|3E343C05EE6FF04D5A54066E19DC6576784E09E7|pta]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAATAAAACCTCCTCAAA, downstream forward: _UP4_TAATAAAATTGAAGACAATG
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-10-15 13:16:30
Author of last update: Christoph.elfmann