mmgD
168
[metabolite|2-methylcitrate] synthase
locus
BSU_24140
Molecular weight
41.95 kDa
pI
6.2
function
mother cell metabolism
product
[metabolite|2-methylcitrate] synthase
essential
no
ec
2.3.3.5
synonyms
mmgD, yqiO, prpC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0372 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,509,654 2,510,772
The protein
Catalyzed reaction/ biological activity
[metabolite|propionyl-CoA] + H2O + [metabolite|oxaloacetate] --> [metabolite|2-methylcitrate] + [metabolite|CoA] [pubmed|28956599]
does also have (albeit weaker) citrate synthase activity [pubmed|28956599]
[metabolite|acetyl-CoA] + H2O + [metabolite|oxaloacetate] --> [metabolite|citrate] + [metabolite|CoA] + H+ (according to UniProt)
Protein family
citrate synthase family (with [protein|6684F6083B001E9FDC5967799B715AE966B83E1D|citA] and [protein|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ], according to UniProt)
Structure
[PDB|3HWK] (from Mycobacterium tuberculosis, 38% identity) [pubmed|25613812]
[AF|P45858]
Paralogous protein(s)
[protein|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ], [protein|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]
Expression and Regulation
Operons
genes
[gene|226100C0AC13BB345DB0F0F309DEB7F91F3770B0|mmgA]-[gene|FB95ABDC8557F26525E8B211595C130A7203ABC0|mmgB]-[gene|F25CDDEBAFC72036664323619F5197D45E86BE9A|mmgC]-[gene|3DB116F7423C31838ED6EE090B94DA5F93A4F40C|mmgD]-[gene|602EAB591A550F68C14F35F6E5F0A91C3B997343|mmgE]-[gene|2F4A025B8E3F14764D24CD7E33F7608C2EB1C42C|mmgF]
description
[Pubmed|8759838]
regulation
expressed early during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|15699190,8759838]
strongly expressed during oligotrophic growth [pubmed|30792386]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|8759838], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,8759838], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
BKE24140 ([gene|3DB116F7423C31838ED6EE090B94DA5F93A4F40C|mmgD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCCTCAACCTCCGTTT, downstream forward: _UP4_AAATCATGAAAGGAGCTGGT
BKK24140 ([gene|3DB116F7423C31838ED6EE090B94DA5F93A4F40C|mmgD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCCTCAACCTCCGTTT, downstream forward: _UP4_AAATCATGAAAGGAGCTGGT
References
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Time of last update: 2024-12-21 11:32:03
Author of last update: Jstuelk